RabMAb

Anti-Phospholipase C beta 3 (phospho S537)抗体[EPR1498Y] (ab51247)

概述

  • 产品名称Anti-Phospholipase C beta 3 (phospho S537)抗体[EPR1498Y]
    参阅全部 Phospholipase C beta 3 一抗
  • 描述
    兔单克隆抗体[EPR1498Y] to Phospholipase C beta 3 (phospho S537)
  • 特异性This antibody detects Phospholipase C beta 3 phosphorylated on Ser537.
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Phosphor-specific peptide corresponding to residues surrounding Serine 537 of human Phospholipase C beta 3.

  • 阳性对照
    • HeLa cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

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应用

Our Abpromise guarantee covers the use of ab51247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
  • 靶标

    • 功能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
    • 序列相似性Contains 1 C2 domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase beta-3 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-beta-3 antibody
      • Phospholipase C beta 3 antibody
      • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
      • Phospholipase C-beta-3 antibody
      • PLC beta 3 antibody
      • PLC-beta-3 antibody
      • Plcb3 antibody
      • PLCB3_HUMAN antibody
      see all

    Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] 图像

    • All lanes : Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247) at 1/2000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 139 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Anti-Phospholipase C beta 3 (phospho S537) antibody [EPR1498Y] (ab51247)参考文献

    ab51247 has not yet been referenced specifically in any publications.

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