Anti-Phospholipase C beta 3 (phospho S537)抗体(ab73998)


  • 产品名称Anti-Phospholipase C beta 3 (phospho S537)抗体
    参阅全部 Phospholipase C beta 3 一抗
  • 描述
    兔多克隆抗体to Phospholipase C beta 3 (phospho S537)
  • 特异性ab73998 detects endogenous levels of Phospholipase C beta 3 only when phosphorylated at serine 537.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    synthesized phosphopeptide derived from human Phospholipase C beta 3 around the phosphorylation site of serine 537 (Q-K-SP-L-G).

  • 阳性对照
    • IHC-P: Human brain tissue WB: Extracts from Jurkat cells treated with UV (15mins) IF/ICC: HCT116 cell line.



Our Abpromise guarantee covers the use of ab73998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 139 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.


  • 功能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase beta-3 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta-3 antibody
    • Phospholipase C beta 3 antibody
    • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
    • Phospholipase C-beta-3 antibody
    • PLC beta 3 antibody
    • PLC-beta-3 antibody
    • Plcb3 antibody
    • PLCB3_HUMAN antibody
    see all

Anti-Phospholipase C beta 3 (phospho S537) antibody 图像

  • ICC/IF image of ab73998 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73998, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • All lanes : Anti-Phospholipase C beta 3 (phospho S537) antibody (ab73998) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells, treated with UV (15mins)
    Lane 2 : Extracts from Jurkat cells, treated with UV (15mins) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 139 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab73998. Left: untreated sample. Right: sample treated with immunising phosphopeptide.

Anti-Phospholipase C beta 3 (phospho S537) antibody (ab73998)参考文献

ab73998 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Human Cell (Hela cells)
Specification Hela cells
Permeabilization Yes - 0,1% Saponin / PBS
Fixative Methanol

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Verified customer

提交于 Oct 09 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH6.0)
Sample Mouse Tissue sections (brain tissue)
Specification brain tissue
Permeabilization No
Fixative Paraformaldehyde

Abcam user community

Verified customer

提交于 Oct 09 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Sample Human Cell lysate - whole cell (Hela cells)
Specification Hela cells
Treatment see additional notes
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Aug 29 2014

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