RabMAb

Anti-Phospholipase C beta 3抗体[EPR5951] (ab124735)

概述

  • 产品名称Anti-Phospholipase C beta 3抗体[EPR5951]
    参阅全部 Phospholipase C beta 3 一抗
  • 描述
    兔单克隆抗体[EPR5951] to Phospholipase C beta 3
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Phospholipase C beta 3.

  • 阳性对照
    • HCT 116, 293T, and A431 cell lysates; Human breast and colon tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab124735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 139 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
    • 序列相似性Contains 1 C2 domain.
      Contains 1 PI-PLC X-box domain.
      Contains 1 PI-PLC Y-box domain.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
      • 1-phosphatidylinositol-4 antibody
      • 5-bisphosphate phosphodiesterase beta-3 antibody
      • Phosphoinositide phospholipase C antibody
      • Phosphoinositide phospholipase C-beta-3 antibody
      • Phospholipase C beta 3 antibody
      • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
      • Phospholipase C-beta-3 antibody
      • PLC beta 3 antibody
      • PLC-beta-3 antibody
      • Plcb3 antibody
      • PLCB3_HUMAN antibody
      see all

    Anti-Phospholipase C beta 3 antibody [EPR5951] 图像

    • All lanes : Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735) at 1/1000 dilution

      Lane 1 : HCT 116 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 139 kDa
    • ab124735 at 1/100 dilution staining Phospholipase C beta 3 in paraffin-embedded Human breast tissue by Immunohistochemistry.
    • ab124735 at 1/100 dilution staining Phospholipase C beta 3 in paraffin-embedded Human colon tissue by Immunohistochemistry.

    Anti-Phospholipase C beta 3 antibody [EPR5951] (ab124735)参考文献

    ab124735 has not yet been referenced specifically in any publications.

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