Anti-Phospholipase C beta 3抗体(ab52199)

概述

  • 产品名称Anti-Phospholipase C beta 3抗体
    参阅全部 Phospholipase C beta 3 一抗
  • 描述
    兔多克隆抗体to Phospholipase C beta 3
  • 特异性This antibody detects endogenous levels of total Phospholipase C beta 3 protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A synthetic non-phosphopeptide designed around the phosphorylation site of serine 1105, of human Phospholipase C beta 3.

  • 阳性对照
    • Human brain tissue (IHC).

性能

应用

Our Abpromise guarantee covers the use of ab52199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 137 kDa (predicted molecular weight: 137 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 序列相似性Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3 antibody
    • 1-phosphatidylinositol-4 antibody
    • 5-bisphosphate phosphodiesterase beta-3 antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta-3 antibody
    • Phospholipase C beta 3 antibody
    • Phospholipase C beta 3 (phosphatidylinositol specific) antibody
    • Phospholipase C-beta-3 antibody
    • PLC beta 3 antibody
    • PLC-beta-3 antibody
    • Plcb3 antibody
    • PLCB3_HUMAN antibody
    see all

Anti-Phospholipase C beta 3 antibody 图像

  • Paraffin-embedded human brain tissue stained for phospholipase C beta 3, using ab52199 (1/100). The right hand panel represents a negative control in which ab52199 has been pre-incubated with the immunizing (blocking) peptide.
  • All lanes : Anti-Phospholipase C beta 3 antibody (ab52199) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with PMA (125ng/ml, 30min).
    Lane 2 : Extracts from HeLa cells treated with PMA (125ng/ml, 30min). For this panel, ab52199 was pre-incubated with the immunizing (blocking) peptide.


    Predicted band size : 137 kDa
    Observed band size : 137 kDa

Anti-Phospholipase C beta 3 antibody (ab52199)参考文献

This product has been referenced in:
  • Béziau DM  et al. Expression of phosphoinositide-specific phospholipase C isoforms in native endothelial cells. PLoS One 10:e0123769 (2015). WB, IHC-P . Read more (PubMed: 25875657) »
  • Feng J  et al. Thromboxane-induced contractile response of human coronary arterioles is diminished after cardioplegic arrest. Ann Thorac Surg 92:829-36 (2011). WB ; Human . Read more (PubMed: 21871266) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Sample Human Cell lysate - whole cell (Hela cells)
Specification Hela cells
Treatment see additional notes
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Sep 01 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer (pH6.0)
Sample Human Tissue sections (lung)
Specification lung
Permeabilization No
Fixative Paraformaldehyde
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提交于 Aug 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"