Anti-Phospholipase C beta 1抗体[K92-3 and K32-3] (ab77743)


  • 产品名称
    Anti-Phospholipase C beta 1抗体[K92-3 and K32-3]
    参阅全部 Phospholipase C beta 1 一抗
  • 描述
    小鼠单克隆抗体[K92-3 and K32-3] to Phospholipase C beta 1
  • 特异性
    ab77743 is specific to Phospholipase C beta 1. It also recognizes the 100 kDa and 41 kDa fragments of Phospholipase C beta 1.1
  • 经测试应用
    适用于: IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Full length native protein (purified) corresponding to Cow Phospholipase C beta 1.
    Database link: P10894

  • 阳性对照
    • Bovine brain membrane preparation. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.



Our Abpromise guarantee covers the use of ab77743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
IP Use at 5-1 µg/mg of lysate.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 138 kDa. PubMed: 24501170


  • 功能
    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
  • 疾病相关
    Epileptic encephalopathy, early infantile, 12
  • 序列相似性
    Contains 1 C2 domain.
    Contains 1 PI-PLC X-box domain.
    Contains 1 PI-PLC Y-box domain.
  • 细胞定位
    Nucleus membrane. Cytoplasm. Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 phosphatidylinositol 4,5 bisphosphate phosphodiesterase beta 1 antibody
    • 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate antibody
    • 1-phosphatidylinositol 4 antibody
    • 5-bisphosphate phosphodiesterase beta-1 antibody
    • EIEE12 antibody
    • Inositoltrisphosphohydrolase antibody
    • Monophosphatidylinositol phosphodiesterase antibody
    • Phosphb antibody
    • Phosphoinositidase C antibody
    • Phosphoinositide phospholipase C antibody
    • Phosphoinositide phospholipase C-beta 1 antibody
    • Phosphoinositide phospholipase C-beta-1 antibody
    • Phospholipase C beta 1 (phosphoinositide-specific) antibody
    • Phospholipase C I antibody
    • Phospholipase C-beta-1 antibody
    • Phospholipase C-I antibody
    • PI PLC antibody
    • PLC 1 antibody
    • PLC beta 1 antibody
    • PLC I antibody
    • PLC-154 antibody
    • PLC-beta-1 antibody
    • PLC-I antibody
    • PLC154 antibody
    • Plcb antibody
    • Plcb1 antibody
    • Plcb1 protein antibody
    • PLCB1_HUMAN antibody
    • PLCbeta1 antibody
    • Triphosphoinositide phosphodiesterase antibody
    see all


  • IP/WB
    5 µg of ab77743 was used to immunoprecipitate Phospholipase C beta 1 from 1 mg of a bovine brain membrane preparation. The immunoprecipitate was resolved by electrophoresis, transferred to nitrocellulose and probed with ab77743 (5 µg/ml). Proteins were visualized using a goat anti mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
    Arrows indicate Phospholipase C beta 1 (150 kDa), heavy (H) and light (L) IgG chains.
  • IHC image of Phospholipase C beta 1 staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77743, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:
  • Zhang CL  et al. Chai-Qin-Cheng-Qi Decoction and Carbachol Improve Intestinal Motility by Regulating Protein Kinase C-Mediated Ca(2+) Release in Colonic Smooth Muscle Cells in Rats with Acute Necrotising Pancreatitis. Evid Based Complement Alternat Med 2017:5864945 (2017). Read more (PubMed: 28529530) »
  • Wright MF  et al. Mechanisms of intracellular calcium homeostasis in developing and mature bovine corpora lutea. Biol Reprod 90:55 (2014). WB ; Cow . Read more (PubMed: 24501170) »

See all 2 Publications for this product


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Thank you for confirming these details and for your cooperation. I can confirm that : - the blocking peptide for ab5804 is ab5839 - the immunogen of ab103278 is aa 1155-1184 of the isoform A (SwissProt: Q9NQ66-1) Because we carry over 70,000 ...

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Thank you for contacting us and for sending images of the results obtained with ab5804 and ab77743. 1)  ab5804 : I looked at the image of the blot and I am positive that the band observed above the 250kDa marker is ITPR1. As explained in my previou...

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