Anti-Phospholipase A2抗体(ab23705)

概述

  • 产品名称
    Anti-Phospholipase A2抗体
  • 描述
    兔多克隆抗体to Phospholipase A2
  • 经测试应用
    适用于: IHC-P, WB, IPmore details
  • 种属反应性
    与反应: Rat, Human
    不与反应: Rabbit, Cow, Pig
  • 免疫原

    Human recombinant sPLA2 (Type IIa).

应用

Our Abpromise guarantee covers the use of ab23705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 14 kDa.
IP Use at an assay dependent concentration.

靶标

  • 功能
    Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
  • 序列相似性
    Belongs to the phospholipase A2 family.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • GIIC sPLA2 antibody
    • Group IIA phospholipase A2 antibody
    • membrane associated antibody
    • MOM1 antibody
    • Non-pancreatic secretory phospholipase A2 antibody
    • NPS-PLA2 antibody
    • OTTHUMP00000002786 antibody
    • PA2GA_HUMAN antibody
    • Phosphatidylcholine 2-acylhydrolase 2A antibody
    • Phospholipase A2 antibody
    • Phospholipase A2 group IIA antibody
    • Phospholipase A2, group IIA (platelets, synovial fluid) antibody
    • PLA2 antibody
    • PLA2B antibody
    • PLA2G2A antibody
    • PLA2L antibody
    • PLA2S antibody
    • PLAS1 antibody
    • RASF A antibody
    • sPLA2 antibody
    see all

图片

  • ab23705 at 1/1000 western blot detection of 0.25 ug of human secretory phospolipase A2.
  • IHC image of ab23705 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23705, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

文献

This product has been referenced in:
  • Wang J  et al. Qishenyiqi Dropping Pill attenuates myocardial fibrosis in rats by inhibiting RAAS-mediated arachidonic acid inflammation. J Ethnopharmacol 176:375-84 (2015). WB ; Rat . Read more (PubMed: 26590099) »
  • Pernet E  et al. Pseudomonas aeruginosa eradicates Staphylococcus aureus by manipulating the host immunity. Nat Commun 5:5105 (2014). IHC ; Human . Read more (PubMed: 25290234) »

See all 5 Publications for this product

客户评价及客户问答

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Gingiva)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Specification
Gingiva
Blocking step
Generic block reagent as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
Formaldehyde
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提交于 Jan 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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