Anti-Cytosolic Phospholipase A2抗体(ab104252)
Key features and details
- Goat polyclonal to Cytosolic Phospholipase A2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-Cytosolic Phospholipase A2抗体
参阅全部 Cytosolic Phospholipase A2 一抗 -
描述
山羊多克隆抗体to Cytosolic Phospholipase A2 -
宿主
Goat -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Horse, Orangutan -
免疫原
Synthetic peptide:
C-QEKTFRQQRKEHIR
, corresponding to internal sequence amino acids 153-166 of Human Phospholipase A2 (NP_077734.1). -
阳性对照
- WB: HeLa cell lysates. ICC/IF: A549 cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab104252 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab104252于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 85 kDa.
1 hour primary incubation is recommended for this product. |
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
|
IHC-P |
Use a concentration of 3.75 µg/ml.
|
说明 |
---|
WB
Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 85 kDa. 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 3.75 µg/ml. |
靶标
-
功能
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response. -
组织特异性
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium. -
序列相似性
Contains 1 C2 domain.
Contains 1 PLA2c domain. -
结构域
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+). -
翻译后修饰
Activated by phosphorylation at both Ser-505 and Ser-727. -
细胞定位
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion. - Information by UniProt
-
数据库链接
- Entrez Gene: 100033889 Horse
- Entrez Gene: 151056 Human
- Entrez Gene: 5321 Human
- Entrez Gene: 100173125 Orangutan
- Entrez Gene: 192259 Rat
- Omim: 600522 Human
- SwissProt: O77793 Horse
- SwissProt: P47712 Human
see all -
别名
- Calcium dependent phospholipid binding protein antibody
- CPLA 2 antibody
- cPLA2 alpha antibody
see all
图片
-
Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation with ab104252 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml) (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
-
Anti-Cytosolic Phospholipase A2 antibody (ab104252) at 1 µg + HeLa cell lysate at 35 µg
Predicted band size: 85 kDa
数据表及文件
-
Datasheet download
文献 (1)
ab104252 被引用在 1 文献中.
- Tunset HM et al. Cytosolic Phospholipase A2 Alpha Regulates TLR Signaling and Migration in Metastatic 4T1 Cells. Int J Mol Sci 20:N/A (2019). PubMed: 31569627