Anti-Phospholipase A2 activator protein (PLAP)抗体[Y102] (ab32354)


  • 产品名称Anti-Phospholipase A2 activator protein (PLAP)抗体[Y102]
    参阅全部 Phospholipase A2 activator protein (PLAP) 一抗
  • 描述
    兔单克隆抗体[Y102] to Phospholipase A2 activator protein (PLAP)
  • 特异性ab32354 recognises Phospholipase A2 activator protein (PLAP).
  • 经测试应用适用于: WB, IHC-P, ICCmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-term of human PLAP.

  • 阳性对照
    • MCF-7 and K562 cell lysates and human lung metastatic cancer tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.


    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号Y102
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab32354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/50 - 1/100.
ICC 1/50.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Involved in the maintenance of ubiquitin levels.
    • 序列相似性Belongs to the WD repeat PLAP family.
      Contains 6 ARM repeats.
      Contains 1 PFU domain.
      Contains 1 PUL domain.
      Contains 7 WD repeats.
    • 结构域The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.
      The PFU domain mediates interaction with ubiquitin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ11281 antibody
      • FLJ12699 antibody
      • Glycerophosphatase antibody
      • OTTHUMP00000045176 antibody
      • Phospholipase A 2 activating protein antibody
      • Phospholipase A-2-activating protein antibody
      • Phospholipase A2 activating protein antibody
      • PLA2P antibody
      • Plaa antibody
      • PLAP antibody
      • PLAP_HUMAN antibody
      see all

    Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] 图像

    • All lanes : Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354) at 1/1000 dilution

      Lane 1 : MCF-7 cell lysate
      Lane 2 : K562 cell lysate

      Predicted band size : 80 kDa
      Observed band size : 81 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human lung metastatic cancer using ab32354 at 1/50 dilution.

    Anti-Phospholipase A2 activator protein (PLAP) antibody [Y102] (ab32354)参考文献

    This product has been referenced in:
    • Zhang F  et al. Alteration in the activation state of new inflammation-associated targets by phospholipase A2-activating protein (PLAA). Cell Signal 20:844-61 (2008). Read more (PubMed: 18291623) »
    • Alexandru G  et al. UBXD7 binds multiple ubiquitin ligases and implicates p97 in HIF1alpha turnover. Cell 134:804-16 (2008). Read more (PubMed: 18775313) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your reply. I would be happy to provide a replacement vial of ab18184. To do so I will just need to access the original order on our system. Please provide the Abcam order reference number or an internal purchase order number so I can pro...

    Read More
    Application Western blot
    Sample Human Cell lysate - whole cell (fibroblasts culture)
    Loading amount 30 µg
    Specification fibroblasts culture
    Gel Running Conditions Reduced Denaturing (10% SDS-page gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Dr. Yishay Shoval

    Verified customer

    提交于 Jan 04 2012

    Thank you for your enquiry. The exact location of the peptide is proprietary information but it is between aa 70 - 90. I hope this helps. Please do not hesitate to contact us if you have any more questions.