Anti-Phospholamban (phospho S16 + T17)抗体(ab62170)

概述

  • 产品名称Anti-Phospholamban (phospho S16 + T17)抗体
    参阅全部 Phospholamban 一抗
  • 描述
    兔多克隆抗体to Phospholamban (phospho S16 + T17)
  • 特异性Detects endogenous levels of Phospholamban only when phosphorylated at serine 16 and threonine 17
  • 经测试应用适用于: WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human Phospholamban around the phosphorylation site of serine 16 and threonine 17 (R-A-SP-TP-I-E).

  • 阳性对照
    • HuvEc cells

性能

应用

Our Abpromise guarantee covers the use of ab62170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 6 kDa. PubMed: 24386101
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

靶标

  • 功能Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • 组织特异性Heart muscle (at protein level).
  • 疾病相关Cardiomyopathy, dilated 1P
    Cardiomyopathy, familial hypertrophic 18
  • 序列相似性Belongs to the phospholamban family.
  • 翻译后修饰Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
  • 细胞定位Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cardiac phospholamban antibody
    • CMD1P antibody
    • CMH18 antibody
    • PLB antibody
    • Pln antibody
    • PPLA_HUMAN antibody
    see all

Anti-Phospholamban (phospho S16 + T17) antibody 图像

  • Immunofluorescence analysis of HuvEc cells, using Phospholamban (phospho S16 + T17) antibody (ab62170) at 1/500 - 1/1000 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.

Anti-Phospholamban (phospho S16 + T17) antibody (ab62170)参考文献

This product has been referenced in:
  • Huang Y  et al. Sarcoplasmic phospholamban protein is involved in the mechanisms of postresuscitation myocardial dysfunction and the cardioprotective effect of nitrite during resuscitation. PLoS One 8:e82552 (2013). WB ; Rat . Read more (PubMed: 24386101) »

See 1 Publication for this product

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