RabMAb

Anti-Phospholamban (phospho S16)抗体[EPR1911] (ab92697)

概述

  • 产品名称Anti-Phospholamban (phospho S16)抗体[EPR1911]
    参阅全部 Phospholamban 一抗
  • 描述
    兔单克隆抗体[EPR1911] to Phospholamban (phospho S16)
  • 经测试应用适用于: WB, IPmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding Serine 16 of Human Phospholamban.

  • 阳性对照
    • Fetal heart lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab92697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: 1/50.
    WB: 1/200,000 - 1/500,000. Detects a band of approximately 11 kDa (predicted molecular weight: 6 kDa).

    Is unsuitable for IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
    • 组织特异性Heart muscle (at protein level).
    • 疾病相关Cardiomyopathy, dilated 1P
      Cardiomyopathy, familial hypertrophic 18
    • 序列相似性Belongs to the phospholamban family.
    • 翻译后修饰Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
    • 细胞定位Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
    • Information by UniProt
    • 数据库链接
    • 别名
      • Cardiac phospholamban antibody
      • CMD1P antibody
      • CMH18 antibody
      • PLB antibody
      • Pln antibody
      • PPLA_HUMAN antibody
      see all

    Anti-Phospholamban (phospho S16) antibody [EPR1911] 图像

    • All lanes : Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697) at 1/200000 dilution

      Lane 1 : Fetal heart lysate (untreated)
      Lane 2 : Fetal heart lysate (treated with LP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

      Predicted band size : 6 kDa

    Anti-Phospholamban (phospho S16) antibody [EPR1911] (ab92697)参考文献

    ab92697 has not yet been referenced specifically in any publications.

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