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Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.
The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine. (Y/F/W)(S*/T*)F
Our Abpromise guarantee covers the use of ab17464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20.
Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
This image is courtesy of an anonymous Abreview
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