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Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.
The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine.
Our Abpromise guarantee covers the use of ab17464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20.
Image courtesy of an anonymous Abreview.The lower blot is to detect total mybpc3. It was probed with a non-abcam anti-mybpc3 antibody.
ab17464 staining Phospho - (Ser/Thr) Phe in murine cardiomyocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized using 1% Triton X-100, blocked with 10% horse serum for 1 hour at room temperature and then incubated with ab17464 at a 1/100 dilution for 2 hours. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
This image is courtesy of an anonymous Abreview