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Antigen is based on the versatile phospho-S6 substrate peptide(KRRRLApSLR). The S6 substrate peptide (KRRRLASLR) is well documented as a substrate for p70 S6K and other kinases such as PKC, PKM and PKA.
Our Abpromise guarantee covers the use of ab9974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Other||Use at an assay dependent concentration.
Immuno-capture assay of kinase: Use immobilized at 5 mg/mL in carbonate buffer (pH8.5-9.0).
|WB||Use a concentration of 2 mg/ml.|
Sensitivity test of anti-pS6 HRP with both phosphorylated and non-phosphorylated S6 peptide substrate.
ab9974 has not yet been referenced specifically in any publications.