Anti-Phosphinotricin Acetyl Transferase抗体- Aminoterminal end (ab50504)

概述

  • 产品名称Anti-Phosphinotricin Acetyl Transferase抗体- Aminoterminal end
  • 描述
    兔多克隆抗体to Phosphinotricin Acetyl Transferase - Aminoterminal end
  • 特异性Ab50504 (N terminal) recognizes bacterial Phosphinotricin Acetyl Transferase expressed in transgenic tobacco plants.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    Reacts with Streptomyces hygroscopicus. Not yet tested in other species.
  • 免疫原

    Synthetic peptide:

    MSPERRPADIRRATEAD

    , corresponding to N terminal amino acids 1-17 of Streptomyces hygroscopicus Phosphinotricin Acetyl Transferase conjugated to KLH.

  • 阳性对照
    • Extract (cytosolic fraction) of leaves from transgenic Nicotiana tabacum expressing the Phosphinotricin Acetyl Transferase gene for Streptomyces hygroscopicus.

性能

应用

Our Abpromise guarantee covers the use of ab50504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
    Staining of the Phosphinotricin Acetyl Transferase band by immunoblotting is specifically inhibited with the Phosphinotricin Acetyl Transferase immunizing peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性The most commonly used selection markers in plant transformation are the bacterial bar gene encoding for the phosphinotricin acetyl transferase (PAT) and the gene encoding for neomycin phosphotransferase II (NPTII). The bar/pat gene was isolated from Streptomyces hygroscopicus. It encodes a small (21 kDa) protein. The pat gene confers resistance to the glufosinate ammonium herbicides bialaphos (BASTA), and phosphinotricin (PPT). PAT belongs to the family of acetyltransferases. It acetylates and inactivates the herbicides bialaphos and PPT. PAT can be used to detect relative transfection efficiencies in cells where multiple transfections are performed in parallel with different plasmids. PAT has been used routinely as a resistance gene in the production of genetically engineered crops and for the production of herbicide resistant crops. The PAT gene is an approved food additive for use as a processing aid in the development of new varieties of crops, including wheat, corn, and rice.
    • 别名
      • PAT antibody
      • Phosphinothricin resistance protein antibody
      • PPT N acetyltransferase antibody

    Anti-Phosphinotricin Acetyl Transferase antibody - Aminoterminal end (ab50504)参考文献

    ab50504 has not yet been referenced specifically in any publications.

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