为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如 Google Chrome
Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab83377) is a simple convenient means of measuring phosphatidylcholine in a variety of biological samples. This assay uses an enzyme-coupled reaction to hydrolyze phosphatidylcholine to release choline, which subsequently oxidizes the OxiRed probe in order to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). This assay measures phosphatidylcholine in the range of 0.1 to 10 nmol per sample.
Visit our FAQs page for tips and troubleshooting.
Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.
PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).
|OxiRed Probe in DMSO||Red||1 x 200µl|
|PC Assay Buffer||WM||1 x 25ml|
|PC Development Mix (Lyophilised)||Green||1 vial|
|PC Hydrolysis Enzyme (lyophilized)||Purple||1 vial|
|PC Standard (10 µmol) (Lyophilised)||Yellow||1 vial|
Our Abpromise guarantee covers the use of ab83377 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Phosphatidycholine levels measured fluorometrically in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).
Phosphatidycholine levels colorimetrically measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
Phosphatidycholine levels colorimetrically measured in rat biological fluids (background signal subtracted, mean of duplicates; +/- SD).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"