Phosphatidylcholine Assay试剂盒(Colorimetric/Fluorometric) (ab83377)

概述

  • 产品名称Phosphatidylcholine Assay试剂盒(Colorimetric/Fluorometric)
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • 检测类型Quantitative
  • 灵敏度
    > 0.2 mM
  • 范围
    0.2 mM - 2.5 mM
  • 检测时间
    0h 40m
  • 产品概述

    Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab83377) is a simple convenient means of measuring phosphatidylcholine in a variety of biological samples. This assay uses an enzyme-coupled reaction to hydrolyze phosphatidylcholine to release choline, which subsequently oxidizes the OxiRed probe in order to generate fluorescence (Ex/Em 535 nm 587 nm) and absorbance (570 nm). This assay measures phosphatidylcholine in the range of 0.1 to 10 nmol per sample.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.

    PC is present in serum at ~0.2-2.5 mM (~50-200 mg/dL).

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 相关性Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid.
  • 别名
    • PC

相关产品

应用

Our Abpromise guarantee covers the use of ab83377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) 图像

  • Phosphatidycholine levels measured fluorometrically in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).

  • Phosphatidycholine levels colorimetrically measured  in cell lysates (background signal subtracted, mean of duplicates; +/- SD).

  • Phosphatidycholine levels colorimetrically measured in rat biological fluids (background signal subtracted, mean of duplicates; +/- SD).

  • Examples of Colorimetric and Fluorometric Phosphatidylcholine standard curves using ab83377.

实验方案

Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric) (ab83377)参考文献

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Product Wall

Yes, there is a mild detergent in the assay buffer.
Here is the tissue homogenization protocol:
Start with 10-100 mg of the tissue, add 500-1000 μl (or ˜4-6 volumes) of the assay buffer on ice, homogenize using a Dounce homogenizer (10-50 pa...

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Thank you for contacting us regarding the issue which you have been experiencing when using ab83377.

Our development team has reviewed this issue and could not find any specific information about interference from this drug. However they sug...

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Thank you for your phone call. 
Any treatment on the cells should be performed before the protocol is begun.  Cell lysates can be prepared using the following protocol:

Start with 2 x 106 cells and suspend the cell pellet in 500 uL of a...

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Thank you for your enquiry regarding ab83377. This kit has been tested and characterized for detecting phosphatidylcholine. At this point we do not have such data (reactivity with lysophosphatidylcholine) and this will have to be specifically determine...

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Thank you for contacting us. The lab has not tested this kit for use on bile samples, but it may be suitable. The bile samples can be stored at -80C prior to use and diluted in the provided assay buffer. I hope this information is helpful to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"