Phosphate Assay试剂盒(Fluorometric) (ab102508)

概述

  • 产品名称Phosphate Assay试剂盒(Fluorometric)
    参阅全部 Phosphate 试剂盒
  • 测试
    100 x 1 assay
  • 样品类型
    Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
  • 检测类型Quantitative
  • 灵敏度
    > 0.1 nmol/well
  • 范围
    2 µM - 10 µM
  • 检测时间
    2h 00m
  • 产品概述

    Abcam's Phosphate Assay Kit (Fluorometric) provides a sensitive, easy, quick means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate reacted with maltose to produce glucose in the present of a special enzyme. The glucose is specifically oxidized to generate a product that reacts with the OxiRed probe to generate fluorescence (Ex/Em=535/587nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples. Glucose background can be eliminated by doing a glucose control.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in variety samples or monitor Pi changes during kinase and phosphatase reactions.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 存放说明Store at -20°C. Please refer to protocols.
  • 组件 标识符 100 tests
    Converter Blue 1 vial
    Developer Green 1 vial
    Phosphate Assay Buffer WM 1 x 25ml
    Phosphate Standard Yellow 1 x 50µl
    Phosphate Substrate Purple 1 x 0.2ml
    PicoProbe™ (in DMSO) Blue 1 x 200µl
  • 研究领域
  • 相关性Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.

应用

Our Abpromise guarantee covers the use of ab102508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

Phosphate Assay Kit (Fluorometric) 图像

  • Phosphate Standard Curve - 2 hours

实验方案

Phosphate Assay Kit (Fluorometric) (ab102508)参考文献

This product has been referenced in:
  • Dai H  et al. Transient binding of an activator BH3 domain to the Bak BH3-binding groove initiates Bak oligomerization. J Cell Biol 194:39-48 (2011). Read more (PubMed: 21727192) »

See 1 Publication for this product

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The laboratory have suggested the following protocol for the sample preparation:

Trypsinize cells and collect. Remove medium + Trypsin.

Add 2-3 volumes of cold assay buffer (200-300 ul buffer for 1...

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ab65622 is a colorimetric assay with a working range of 1-10uM concentration of phosphate. ab65622 provides enough materials to carry out 500 assays.

ab102508 is a more sensitive fluorometric...

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Thank you for contacting us.
The phosphate assay buffer, probe, converter and developer does not contain any known free interfering phosphate ions, if they does then blank control is to be used anyway, the reading of which will be deduced as backg...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"