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Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method for assessing phosphate over a wide range of concentrations. The assay utilizes a proprietary formulation of malachite green and ammonimum molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around OD = 650nm.
This assay can be used not only in biological fluids but also in inorganic samples such as algal blooms and water from run-off areas of high fertilizer use.
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Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.
|Phosphate Reagent||1 x 15ml|
|Phosphate Standard (10 mM)||1 x 500µl|
Our Abpromise guarantee covers the use of ab65622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
Phosphate measured in tissue lysates showing quantity (µmol) per mg of extracted protein.
Protein concentration for samples varied from 9 mg/mL to 14 mg/mL. Samples were diluted 400-4000 fold.
Phosphate measured in cell culture lysates showing quantity (µmol) per mg of extracted protein.
Samples with the concentration of 1.3e7 cells/mL were used. Samples were diluted 400-4000 fold.
Phosphate measured in biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 100-1000 fold.