Horse, Cow, Cat, Dog, Human
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PHOS.
(Peptide available as
This antibody gave a positive signal in the following tissue lysates: Mouse Retina; Rat Retina; Rat Eye.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Use a concentration of 1 µg/ml.
May participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. Inhibits the transcriptional activation activity of the cone-rod homeobox CRX.
Belongs to the phosducin family.
Light-induced changes in cyclic nucleotide levels modulate the phosphorylation of this protein by cAMP kinase.
Nucleus and Cytoplasm > cytosol. Cell projection > cilium > photoreceptor outer segment. Photoreceptor inner segment. Nucleus. Outer and inner segments of the rod cells.
Information by UniProt
33 kDa phototransducing protein antibody
MEKA protein antibody
Western blot - Anti-PHOS antibody (ab77523)
All lanes : Anti-PHOS antibody (ab77523) at 1 µg/ml Lane 1 : Mouse Retina Tissue Lysate Lane 2 : Rat Retina Tissue Lysate Lane 3 : Rat Eye Tissue Lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 28 kDa Observed band size: 28 kDa Exposure time: 6 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHOS antibody (ab77523)
IHC image of ab77523 staining in Mouse Eye formalin fixed paraffin embedded tissue section, performed on a Leica Bond
TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77523, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"