- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-PHLPP2抗体
参阅全部 PHLPP2 一抗 -
描述兔多克隆抗体to PHLPP2
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宿主Rabbit
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经测试应用适用于: ICC/IF, WB, IPmore details
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种属反应性与反应: Mouse, Human
预测可用于: Rabbit, Guinea pig, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide corresponding to a region between residues 1275 and the C-terminus (residue 1323) of human PHLPP2 (NP_055835.2)
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阳性对照
- Whole cell lysates from HeLa, 293T or NIH/3T3 cells. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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存储溶液Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab71973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 147 kDa). | |
| IP | Use at 1-4 µg/mg of lysate. |
靶标
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功能Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
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组织特异性In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).
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序列相似性Contains 22 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain. -
细胞定位Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 23035 Human
- Entrez Gene: 244650 Mouse
- Omim: 611066 Human
- SwissProt: Q6ZVD8 Human
- SwissProt: Q8BXA7 Mouse
- Unigene: 709458 Human
- Unigene: 23494 Mouse
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别名
- KIAA0931 antibody
- PH domain and leucine rich repeat protein phosphatase 2 antibody
- PH domain leucine rich repeat protein phosphatase like antibody
see all
图片
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Western blot - Anti-PHLPP2 antibody (ab71973)All lanes : Anti-PHLPP2 antibody (ab71973) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 147 kDa
Observed band size: 190 kDa (why is the actual band size different from the predicted?) -
Immunoprecipitation - Anti-PHLPP2 antibody (ab71973)Detection of PHLPP2 by Western Blot of Immunoprecipitate.
ab71973 at 1µg/ml staining PHLPP2 in HeLa whole cell lysate immunoprecipitated using ab71973 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds. -
Immunocytochemistry/ Immunofluorescence - Anti-PHLPP2 antibody (ab71973)ab71973 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71973 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
文献
This product has been referenced in:
- Castellanos-Martín A et al. Unraveling heterogeneous susceptibility and the evolution of breast cancer using a systems biology approach. Genome Biol 16:40 (2015). Read more (PubMed: 25853295) »
- Liao WT et al. microRNA-224 promotes cell proliferation and tumor growth in human colorectal cancer by repressing PHLPP1 and PHLPP2. Clin Cancer Res 19:4662-72 (2013). Read more (PubMed: 23846336) »
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