Anti-PHLPP抗体- Aminoterminal end (ab71277)

概述

  • 产品名称Anti-PHLPP抗体- Aminoterminal end
    参阅全部 PHLPP 一抗
  • 描述
    兔多克隆抗体to PHLPP - Aminoterminal end
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 阳性对照
    • K562 cell line lysates.

性能

应用

Our Abpromise guarantee covers the use of ab71277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/1000.
    WB: 1/50 - 1/100. Detects bands of approximately 72 and 185 kDa (predicted molecular weight: 185 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts.
    • 组织特异性In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.
    • 序列相似性Contains 21 LRR (leucine-rich) repeats.
      Contains 1 PH domain.
      Contains 1 PP2C-like domain.
    • 结构域The PH domain is required for interaction with PRKCB and its dephosphorylation.
    • 细胞定位Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • hSCOP antibody
      • KIAA0606 antibody
      • PH domain and leucine rich repeat protein phosphatase antibody
      • PH domain leucine rich repeat containing protein phosphatase antibody
      • PH domain leucine rich repeat protein phosphatase antibody
      • PH domain leucine-rich repeat-containing protein phosphatase 1 antibody
      • PH domain-containing family E member 1 antibody
      • PHLP1_HUMAN antibody
      • PHLPP antibody
      • Phlpp1 antibody
      • Pleckstrin homology domain containing family E (with leucine rich repeats) member 1 antibody
      • Pleckstrin homology domain containing family E protein 1 antibody
      • Pleckstrin homology domain-containing family E member 1 antibody
      • PLEKHE 1 protein antibody
      • PLEKHE1 antibody
      • SCN circadian oscillatory protein antibody
      • SCOP antibody
      • Suprachiasmatic nucleus circadian oscillatory protein antibody
      • Suprachiasmatic nucleus circadian protein antibody
      see all

    Anti-PHLPP antibody - Aminoterminal end 图像

    • Anti-PHLPP antibody - Aminoterminal end (ab71277) at 1/60 dilution + K562 cell line lysate at 35 µg

      Predicted band size : 185 kDa
      Observed band size : 185 kDa
      Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.

    Anti-PHLPP antibody - Aminoterminal end (ab71277)参考文献

    ab71277 has not yet been referenced specifically in any publications.

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