概述

  • 产品名称Anti-PHLPP抗体
    参阅全部 PHLPP 一抗
  • 描述
    兔多克隆抗体to PHLPP
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Chicken, Dog, Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Human PHLPP.

  • 阳性对照
    • This antibody gave a positive signal in Human brain and Human brain membrane tissue lysates.

性能

应用

Our Abpromise guarantee covers the use of ab89833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 185 kDa (predicted molecular weight: 185 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts.
  • 组织特异性In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 78% of tested tumors (at protein level). Ubiquitously expressed in non-cancerous tissues.
  • 序列相似性Contains 21 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • 结构域The PH domain is required for interaction with PRKCB and its dephosphorylation.
  • 细胞定位Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated at the lateral membrane of epithelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hSCOP antibody
    • KIAA0606 antibody
    • PH domain and leucine rich repeat protein phosphatase antibody
    • PH domain leucine rich repeat containing protein phosphatase antibody
    • PH domain leucine rich repeat protein phosphatase antibody
    • PH domain leucine-rich repeat-containing protein phosphatase 1 antibody
    • PH domain-containing family E member 1 antibody
    • PHLP1_HUMAN antibody
    • PHLPP antibody
    • Phlpp1 antibody
    • Pleckstrin homology domain containing family E (with leucine rich repeats) member 1 antibody
    • Pleckstrin homology domain containing family E protein 1 antibody
    • Pleckstrin homology domain-containing family E member 1 antibody
    • PLEKHE 1 protein antibody
    • PLEKHE1 antibody
    • SCN circadian oscillatory protein antibody
    • SCOP antibody
    • Suprachiasmatic nucleus circadian oscillatory protein antibody
    • Suprachiasmatic nucleus circadian protein antibody
    see all

Anti-PHLPP antibody 图像

  • All lanes : Anti-PHLPP antibody (ab89833) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Human brain normal tissue lysate - membrane extract (ab29456)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 185 kDa
    Observed band size : 185 kDa


    Exposure time : 20 minutes
  • ICC/IF image of ab89833 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab89833 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PHLPP antibody (ab89833)参考文献

ab89833 has not yet been referenced specifically in any publications.

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