Anti-PHLDA3抗体[4B6] (ab81464)
Key features and details
- Mouse monoclonal [4B6] to PHLDA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
概述
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产品名称
Anti-PHLDA3抗体[4B6]
参阅全部 PHLDA3 一抗 -
描述
小鼠单克隆抗体[4B6] to PHLDA3 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide: MTAAATATVL KEGVLEKRSG GLLQLWKRKR C, corresponding to N terminal amino acids 1-31 of human PHLDA3
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阳性对照
- MDA-MB-468 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Ion Exchange Chromatography -
纯化说明
The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. The antibody is filter sterilized. -
克隆
单克隆 -
克隆编号
4B6 -
同种型
IgG2b -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81464于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
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说明 |
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WB
Use at an assay dependent dilution. Predicted molecular weight: 14 kDa. |
靶标
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功能
p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. -
组织特异性
Widely expressed with lowest expression in liver and spleen. -
序列相似性
Belongs to the PHLDA3 family.
Contains 1 PH domain. -
结构域
The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling. -
细胞定位
Cytoplasm. Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23612 Human
- Entrez Gene: 27280 Mouse
- Entrez Gene: 363989 Rat
- Omim: 607054 Human
- SwissProt: Q9Y5J5 Human
- SwissProt: Q9WV95 Mouse
- SwissProt: Q5PQT7 Rat
- Unigene: 268557 Human
see all -
别名
- PHLA3_HUMAN antibody
- PHLDA 3 antibody
- phlda3 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab81464 被引用在 2 文献中.
- Wang P et al. Pleckstrin homology-like domain family A, member 3, a miR-19a-3p-regulated gene, suppresses tumor growth in osteosarcoma by downregulating the Akt pathway. Bioengineered 13:3993-4009 (2022). PubMed: 35112982
- Prudovsky I et al. Phosphatidylserine colocalizes with epichromatin in interphase nuclei and mitotic chromosomes. Nucleus 3:200-10 (2012). PubMed: 22555604