Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide corresponding to a region between residue 1600 and 1650 of Human PHIP (NP_060404.3).
- Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/2000 - 1/10000. Detects a band of approximately 230 kDa (predicted molecular weight: 207 kDa).
Is unsuitable for IP.
PHIP contains two bromo domains and eight WD repeats. It may be involved in insulin signaling. It is expressed in myeloma and epidermoid carcinoma cell lines.
- DCAF14 antibody
- DDB1 and CUL4 associated factor 14 antibody
- FLJ20705 antibody
Western blot - Anti-PHIP antibody (ab86244)
All lanes :
Anti-PHIP antibody (ab86244) at 0.04 µg/mlLane 1 :
Whole cell lysate from HeLa cells at 50 µgLane 2 :
Whole cell lysate from HeLa cells at 15 µgLane 3 :
Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL technique.Predicted band size:
207 kDaObserved band size:
230 kDa (why is the actual band size different from the predicted?
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