概述

  • 产品名称Anti-PHIP抗体
    参阅全部 PHIP 一抗
  • 描述
    小鼠单克隆抗体to PHIP
  • 经测试应用适用于: WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1599-1706 of Human PHIP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2b
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab57522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性PHIP contains two bromo domains and eight WD repeats. It may be involved in insulin signaling. It is expressed in myeloma and epidermoid carcinoma cell lines.
  • 数据库链接
  • 别名
    • DCAF14 antibody
    • DDB1 and CUL4 associated factor 14 antibody
    • FLJ20705 antibody
    • FLJ45918 antibody
    • IRS 1 PH domain binding protein antibody
    • IRS1 PH domain binding protein antibody
    • MGC90216 antibody
    • Ndrp antibody
    • Neuronal differentiation related protein antibody
    • PH interacting protein antibody
    • Pleckstrin homology domain interacting protein antibody
    • WD repeat containing protein 11 antibody
    • WD repeat protein 11 antibody
    • WDR11 antibody
    see all

Anti-PHIP antibody 图像

  • Western blot against tagged recombinant protein immunogen using ab57522 PHIP antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
  • Overlay histogram showing HeLa cells stained with ab57522 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57522, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PHIP antibody (ab57522)参考文献

ab57522 has not yet been referenced specifically in any publications.

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