概述

  • 产品名称Anti-PHF20抗体
    参阅全部 PHF20 一抗
  • 描述
    兔多克隆抗体to PHF20
  • 经测试应用适用于: IHC-P, IPmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 900-950 (PGSPKVKEYVS KKALPEEAPA RKLLDRGGEG LLSSQHQWQF NLLTHVESLQ) of Human PHF20 (NP_057520.2).

  • 阳性对照
    • HeLa cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab91456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 5-15 µg/mg of lysate.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 功能Possible transcription factor.
    • 组织特异性Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
    • 序列相似性Contains 1 A.T hook DNA-binding domain.
      Contains 1 C2H2-type zinc finger.
      Contains 1 PHD-type zinc finger.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C20orf104 antibody
      • GLEA2 antibody
      • Glioma-expressed antigen 2 antibody
      • HCA58 antibody
      • Hepatocellular carcinoma-associated antigen 58 antibody
      • Novel zinc finger protein antibody
      • NZF antibody
      • PHD finger protein 20 antibody
      • PHF20 antibody
      • PHF20_HUMAN antibody
      • Transcription factor TZP antibody
      • TZP antibody
      see all

    Anti-PHF20 antibody 图像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling PHF20 with ab91456 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Human PHF20 by Western Blot of Immunoprecipitates.
      Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
      ab91456 was used for IP at 10 µg/mg lysate (lane 1), with a control IgG in lane 2. For blotting immunoprecipitates, an anti-PHF20 was used at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-PHF20 antibody (ab91456)参考文献

    ab91456 has not yet been referenced specifically in any publications.

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