The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 5-15 µg/mg of lysate.
应用说明Is unsuitable for WB.
功能Possible transcription factor.
组织特异性Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling PHF20 with ab91456 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - PHF20 antibody (ab91456)
Detection of Human PHF20 by Western Blot of Immunoprecipitates.
Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
ab91456 was used for IP at 10 µg/mg lysate (lane 1), with a control IgG in lane 2. For blotting immunoprecipitates, an anti-PHF20 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Anti-PHF20 antibody (ab91456)参考文献
has not yet been referenced specifically in any publications.