The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IP: Use at 5-15µg/mg of lysate.
WB: 1/2000 - 1/10000. Predicted molecular weight: 115 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Possible transcription factor.
组织特异性Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
All lanes : Anti-PHF20 antibody (ab91434) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique
Predicted band size : 115 kDa
Exposure time : 3 minutes
Immunoprecipitation - PHF20 antibody (ab91434)
1mg whole cell lysate from HeLa cells was immunoprecipitated using 10µg/mg ab91434. 20% IP was loaded per lane, and probed with ab91434 at 1µg/ml (lane 1) or a control IgG (lane 2). Detection: chemoluminescence with exposure time of 30 sec.
Anti-PHF20 antibody (ab91434)参考文献
has not yet been referenced specifically in any publications.