概述

  • 产品名称Anti-PHF20抗体
    参阅全部 PHF20 一抗
  • 描述
    兔多克隆抗体to PHF20
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide designed within residues:

    ETDHKSLSSSP DKREKFKEQR KATVNVKKDK EDKPLKTEKR PKQPDKEGKL

    , corresponding to amino acids 150-200 of Human PHF20 (NP_057520.2).

  • 阳性对照
    • HeLa cell lysate

性能

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应用

Our Abpromise guarantee covers the use of ab91434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
  • 应用说明IP: Use at 5-15µg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 115 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Possible transcription factor.
    • 组织特异性Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
    • 序列相似性Contains 1 A.T hook DNA-binding domain.
      Contains 1 C2H2-type zinc finger.
      Contains 1 PHD-type zinc finger.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • C20orf104 antibody
      • GLEA2 antibody
      • Glioma-expressed antigen 2 antibody
      • HCA58 antibody
      • Hepatocellular carcinoma-associated antigen 58 antibody
      • Novel zinc finger protein antibody
      • NZF antibody
      • PHD finger protein 20 antibody
      • PHF20 antibody
      • PHF20_HUMAN antibody
      • Transcription factor TZP antibody
      • TZP antibody
      see all

    Anti-PHF20 antibody 图像

    • All lanes : Anti-PHF20 antibody (ab91434) at 0.1 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Developed using the ECL technique

      Predicted band size : 115 kDa


      Exposure time : 3 minutes
    • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 10µg/mg ab91434.
      20% IP was loaded per lane, and probed with ab91434 at 1µg/ml (lane 1) or a control IgG (lane 2).
      Detection: chemoluminescence with exposure time of 30 sec.

    Anti-PHF20 antibody (ab91434)参考文献

    ab91434 has not yet been referenced specifically in any publications.

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