概述

  • 产品名称Anti-PHF19抗体
    参阅全部 PHF19 一抗
  • 描述
    小鼠多克隆抗体to PHF19
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    PHF19 (NP_001009936.1, 1 a.a. ~ 207 a.a) full-length human protein.

  • 阳性对照
    • transfected 293T cell lysate; pancreas lysate.

性能

应用

Our Abpromise guarantee covers the use of ab68959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 65 kDa.

靶标

  • 功能Acts as a transcritpional repressor. Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter.
  • 组织特异性Isoform 1 is expressed in thymus, heart, lung and kidney. Isoform 2 is predominantly expressed in placenta, skeletal muscle and kidney, whereas isoform 1 is predominantly expressed in liver and peripheral blood leukocytes. Overexpressed in many types of cancers, including colon, skin, lung, rectal, cervical, uterus, liver cancers, in cell lines derived from different stages of melanoma and in glioma cell lines.
  • 序列相似性Contains 2 PHD-type zinc fingers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPCL3 antibody
    • MGC131698 antibody
    • MGC149712 antibody
    • MGC149713 antibody
    • MGC23929 antibody
    • MTF2L1 antibody
    • OTTHUMP00000021996 antibody
    • OTTHUMP00000021998 antibody
    • PCL3 antibody
    • PHD finger protein 19 antibody
    • Phf19 antibody
    • PHF19 protein antibody
    • PHF19_HUMAN antibody
    • Polycomb like 3 antibody
    • Polycomb like protein 3 antibody
    • Polycomb-like protein 3 antibody
    • TDRD19B antibody
    • Tudor domain containing 19B antibody
    see all

Anti-PHF19 antibody 图像

  • All lanes : Anti-PHF19 antibody (ab68959) at 1/500 dilution

    Lane 1 : transfected 293T cell lysate
    Lane 2 : non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 65 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence using ab68959 on HeLa cells [antibody concentration 10 ug/ml].

Anti-PHF19 antibody (ab68959)参考文献

This product has been referenced in:
  • Ghislin S  et al. PHF19 and Akt control the switch between proliferative and invasive states in melanoma. Cell Cycle 11:1634-45 (2012). Read more (PubMed: 22487681) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. I have had a chance to speak with the lab regarding your question. They have found 3 isoforms of PHF19 protein all contain the immunogen a.a. sequence used to produce the ab68959.(http://www.uniprot.org/uniprot/Q5T6S3 )...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"