概述

  • 产品名称Anti-PHF12抗体
  • 描述
    兔多克隆抗体to PHF12
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 864-913 (VDNVLYSCDF SEKTPPTPPS SIVAKVQSVI RRRRHQKQDE EPSEEAAMMS) of Human PHF12 (NP_001028733).

  • 阳性对照
    • Human fetal brain lysate.

性能

应用

Our Abpromise guarantee covers the use of ab81249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

靶标

  • 相关性PHF12 acts as a transcriptional repressor. It is involved in recruitment of functional SIN3A complexes to DNA. PHF12 represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). It may also repress transcription in a SIN3A-independent manner through recruitment of functional AES complexes to DNA.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • FLJ34122 antibody
    • KIAA1523 antibody
    • MGC131914 antibody
    • Pf1 antibody
    • PHD factor 1 antibody
    • PHD finger protein 12 antibody
    • PHD zinc finger transcription factor antibody
    • PHF 12 antibody
    see all

Anti-PHF12 antibody 图像

  • Anti-PHF12 antibody (ab81249) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 110 kDa
    Observed band size : 110 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

Anti-PHF12 antibody (ab81249)参考文献

ab81249 has not yet been referenced specifically in any publications.

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