重组Anti-PHD finger蛋白1/PHF1抗体[EPR14222] (ab184951)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14222] to PHF1
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PHD finger蛋白1/PHF1抗体[EPR14222]
参阅全部 PHD finger protein 1/PHF1 一抗 -
描述
兔单克隆抗体[EPR14222] to PHF1 -
宿主
Rabbit -
特异性
This antibody is specific to PHD finger protein 1, not paired helical filaments.
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经测试应用
适用于: Flow Cyt (Intra), IHC-P, ICC/IF, WB, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- 293, Jurkat, MCF7 and Human fetal brain lysates; Human pancreas and Rat skeletal muscle tissues; MCF7 cells; 293 cells. Mouse and rat brain tissue lysate. NIH/3T3, RAW264.7, C6 and PC-12 whole cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14222 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184951于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/160.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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WB |
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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IP |
1/30.
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说明 |
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Flow Cyt (Intra)
1/160. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
IP
1/30. |
靶标
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功能
Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex. -
组织特异性
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney. -
序列相似性
Contains 2 PHD-type zinc fingers. -
细胞定位
Nucleus. Localizes specifically to the promoters of numerous target genes. - Information by UniProt
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数据库链接
- Entrez Gene: 5252 Human
- Entrez Gene: 21652 Mouse
- Entrez Gene: 294287 Rat
- Omim: 602881 Human
- SwissProt: O43189 Human
- SwissProt: Q9Z1B8 Mouse
- Unigene: 166204 Human
- Unigene: 480590 Mouse
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别名
- hPCl1 antibody
- MTF2 L2 antibody
- MTF2L 2 antibody
see all
图片
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All lanes : Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951) at 1/1000 dilution
Lane 1 : Mouse brain tissue
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 62 kDa -
PHF1 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab184951 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab184951 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab184951 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184951 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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All lanes : Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951) at 1/20000 dilution
Lane 1 : 293 lysate
Lane 2 : Jurkat lysate
Lane 3 : MCF7 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa -
Anti-PHD finger protein 1/PHF1 antibody [EPR14222] (ab184951) at 1/5000 dilution + Human fetal brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 62 kDa -
Immunofluorescent analysis of MCF7 cells (4% paraformaldehyde-fixed) labeling PHF1 with ab184951 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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Intracellular flow cytometric analysis of 293 cells (2% paraformaldehyde-fixed) labeling PHF1with ab184951 at 1/160 dilution (red) mpared to a negative control antibody (red)or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PHF1 with ab184951 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (4)
ab184951 被引用在 4 文献中.
- Ning J et al. Down-regulated m6A reader FTO destabilizes PHF1 that triggers enhanced stemness capacity and tumor progression in lung adenocarcinoma. Cell Death Discov 8:354 (2022). PubMed: 35945194
- Alexandrova E et al. Interaction Proteomics Identifies ERbeta Association with Chromatin Repressive Complexes to Inhibit Cholesterol Biosynthesis and Exert An Oncosuppressive Role in Triple-negative Breast Cancer. Mol Cell Proteomics 19:245-260 (2020). PubMed: 31792072
- Qu Y et al. PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation. Cell Cycle 17:2447-2459 (2018). PubMed: 30382790
- Noël A et al. Sex-specific lung functional changes in adult mice exposed only to second-hand smoke in utero. Respir Res 18:104 (2017). WB ; Mouse . PubMed: 28651580