Anti-PHD finger蛋白1/PHF1抗体(ab80042)
Key features and details
- Rabbit polyclonal to PHD finger protein 1/PHF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PHD finger蛋白1/PHF1抗体
参阅全部 PHD finger protein 1/PHF1 一抗 -
描述
兔多克隆抗体to PHD finger蛋白1/PHF1 -
宿主
Rabbit -
特异性
This antibody is specific to PHD finger protein 1. From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab184951.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Cynomolgus monkey, Macaque monkey -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in Human Heart Tissue Lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab80042于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex. -
组织特异性
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney. -
序列相似性
Contains 2 PHD-type zinc fingers. -
细胞定位
Nucleus. Localizes specifically to the promoters of numerous target genes. - Information by UniProt
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数据库链接
- Entrez Gene: 508957 Cow
- Entrez Gene: 5252 Human
- Entrez Gene: 21652 Mouse
- Entrez Gene: 294287 Rat
- Omim: 602881 Human
- SwissProt: O43189 Human
- SwissProt: Q9Z1B8 Mouse
- Unigene: 166204 Human
see all -
别名
- hPCl1 antibody
- MTF2 L2 antibody
- MTF2L 2 antibody
see all
图片
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Anti-PHD finger protein 1/PHF1 antibody (ab80042) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Additional bands at: 50 kDa (possible isoform)
Exposure time: 3 minutes
This antibody is predicted to cross react with both isoform 1 (50-kDa) and isoform 2 (62-kDa) of PHF1. We believe that the band observed at 50-kDa corresponds to isoform 1. -
ICC/IF image of ab80042 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80042, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab80042 被引用在 5 文献中.
- Dong C et al. Structural basis for histone variant H3tK27me3 recognition by PHF1 and PHF19. Elife 9:N/A (2020). PubMed: 32869745
- Wang L et al. Deep cervical lymph node ligation aggravates AD-like pathology of APP/PS1 mice. Brain Pathol 29:176-192 (2019). PubMed: 30192999
- Zhang R et al. Aquaporin 4 deletion exacerbates brain impairments in a mouse model of chronic sleep disruption. CNS Neurosci Ther N/A:N/A (2019). PubMed: 31364823
- Qu Y et al. PHF1 is required for chromosome alignment and asymmetric division during mouse meiotic oocyte maturation. Cell Cycle 17:2447-2459 (2018). PubMed: 30382790
- Liu R et al. PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis. Nucleic Acids Res 46:6608-6626 (2018). PubMed: 29846670