概述

  • 产品名称Anti-PHF1抗体[2G7]
    参阅全部 PHF1 一抗
  • 描述
    小鼠单克隆抗体[2G7] to PHF1
  • 经测试应用适用于: WB, ELISA, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Horse, Cow, Dog, Rhesus monkey
  • 免疫原

    Recombinant fragment corresponding to the N terminal amino acids 2-101 (AQPPRLSRSG ASSLWDPASP APTSGPRPRL WEGQDVLARW TDGLLYLGTI KKVDSAREVC LVQFEDDSQF LVLWKDISPA ALPGEELLCC VCRSETVVP) of Human PHF1 (NP_077084) with a proprietary tag.

  • 阳性对照
    • HeLa cells and lysate. Rat adrenal gland pheochromocytoma (PC12).

性能

应用

Our Abpromise guarantee covers the use of ab87985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 62 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
  • 组织特异性Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
  • 序列相似性Contains 2 PHD-type zinc fingers.
  • 细胞定位Nucleus. Localizes specifically to the promoters of numerous target genes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hPCl1 antibody
    • MTF2 L2 antibody
    • MTF2L 2 antibody
    • MTF2L2 antibody
    • PCL 1 antibody
    • PCL1 antibody
    • PHD finger protein 1 antibody
    • PHD finger protein1 antibody
    • PHF 1 antibody
    • PHF 2 antibody
    • Phf1 antibody
    • PHF1_HUMAN antibody
    • PHF2 antibody
    • Plc 1 antibody
    • Plc1 antibody
    • Polycomb like protein 1 antibody
    • Polycomb like protein1 antibody
    • Polycomb-like protein 1 antibody
    • Protein PHF1 antibody
    • T complex testis expressed 3 antibody
    • T complex testis expressed3 antibody
    • Tctex 3 antibody
    • Tctex3 antibody
    see all

Anti-PHF1 antibody [2G7] 图像

  • Overlay histogram showing HeLa cells stained with ab87985 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab82247, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-PHF1 antibody [2G7] (ab87985) at 1 µg/ml + Immunogen at 0.1 µg

    Secondary
    Goat anti mouse HRP at 5000
    Developed using the ECL technique

    Predicted band size : 62 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Expected size of immunogen is 37 kDa.
  • Anti-PHF1 antibody [2G7] (ab87985) at 1 µg/ml + PC12 cell lysate at 25 µg

    Secondary
    Goat anti mouse HRP at 2500
    Developed using the ECL technique

    Predicted band size : 62 kDa
    Observed band size : ~50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : ~70 kDa. We are unsure as to the identity of these extra bands.
  • Anti-PHF1 antibody [2G7] (ab87985) at 1 µg/ml + HeLa NE cell lysate at 25 µg

    Secondary
    Goat anti mouse HRP at 5000
    Developed using the ECL technique

    Predicted band size : 62 kDa
    Observed band size : ~50 kDa (why is the actual band size different from the predicted?)
  • ab87985 at 10 µg/ml detecting PHF1 in paraformaldehyde fixed, permeabilised HeLa cells by immmunofluorescence.
  • ab87985 capturing recombinant GST tagged PHF1 by ELISA. The detection limit for recombinant GST tagged PHF1 is approximately 1ng/ml as a capture antibody.

Anti-PHF1 antibody [2G7] (ab87985)参考文献

ab87985 has not yet been referenced specifically in any publications.

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