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Anti-PHD2 / prolyl hydroxylase抗体[mAbcam74796] (ab74796)

概述

  • 产品名称Anti-PHD2 / prolyl hydroxylase抗体[mAbcam74796]
    参阅全部 PHD2 / prolyl hydroxylase 一抗
  • 描述
    小鼠单克隆抗体[mAbcam74796] to PHD2 / prolyl hydroxylase
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human PHD2/ prolyl hydroxylase.

    (Peptide available as ab94889.)

  • 阳性对照
    • This antibody gave a positive signal in human skeletal muscle tissue lysate, and in the following whole cell lysates: HepG2; HeLa; U20S.

性能

应用

Our Abpromise guarantee covers the use of ab74796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 46 kDa).

靶标

  • 功能Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • 组织特异性According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.
  • 疾病相关Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.
  • 序列相似性Contains 1 Fe2OG dioxygenase domain.
    Contains 1 MYND-type zinc finger.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C1ORF12 antibody
    • Chromosome 1 Open Reading Frame 12 antibody
    • DKFZp761F179 antibody
    • ECYT 3 antibody
    • ECYT3 antibody
    • EGL 9 homolog of C. elegans 1 antibody
    • EGL nine (C.elegans) homolog 1 antibody
    • Egl nine homolog 1 (C. elegans) antibody
    • Egl nine homolog 1 antibody
    • Egl nine like protein 1 antibody
    • EGLN 1 antibody
    • Egln1 antibody
    • EGLN1_HUMAN antibody
    • HIF PH2 antibody
    • HIF Prolyl Hydroxylase 2 antibody
    • HIF-PH2 antibody
    • HIF-prolyl hydroxylase 2 antibody
    • HIFP4H 2 antibody
    • HIFPH2 antibody
    • HPH 2 antibody
    • HPH-2 antibody
    • HPH2 antibody
    • Hypoxia inducible factor prolyl hydroxylase 2 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 2 antibody
    • ORF13 antibody
    • P4H2 antibody
    • PHD 2 antibody
    • PhD2 antibody
    • PNAS 118 antibody
    • PNAS 137 antibody
    • Prolyl Hydroxylase Domain Containing Protein 2 antibody
    • Prolyl hydroxylase domain-containing protein 2 antibody
    • Rat Homolog of SM20 antibody
    • SM 20 antibody
    • SM-20 antibody
    • SM20 antibody
    • Zinc finger MYND domain containing protein 6 antibody
    • ZMYND6 antibody
    see all

Anti-PHD2 / prolyl hydroxylase antibody [mAbcam74796] 图像



  • Predicted band size : 46 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PHD2 / prolyl hydroxylase knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab74796 observed at 52 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab74796 was shown to recognize PHD2 / prolyl hydroxylase when PHD2 / prolyl hydroxylase knockout samples were used, along with additional cross-reactive bands. Wild-type and PHD2 / prolyl hydroxylase knockout samples were subjected to SDS-PAGE. ab74796 at a concentration of 5 µg/ml and 181602 (loading control to GAPDH) diluted to 1/10,000 were incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • All lanes : Anti-PHD2 / prolyl hydroxylase antibody [mAbcam74796] (ab74796) at 5 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 44,52 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
    The 52-kDa band observed is comparable to the band detected by other commercially available antibodies to human prolyl hydroxylase (PHD2). Based on the immunogen sequence, this antibody is predicted to cross react with both isoform 1 (46 kDa) and isoform 2 (44 kDa) of PHD2. We believe that the band observed around 44-kDa corresponds to isoform 2.

Anti-PHD2 / prolyl hydroxylase antibody [mAbcam74796] (ab74796)参考文献

ab74796 has not yet been referenced specifically in any publications.

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