Anti-PHD2 / prolyl hydroxylase抗体- C-terminal (ab226890)
Key features and details
- Rabbit polyclonal to PHD2 / prolyl hydroxylase - C-terminal
- Suitable for: ICC/IF, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-PHD2 / prolyl hydroxylase抗体- C-terminal
参阅全部 PHD2 / prolyl hydroxylase 一抗 -
描述
兔多克隆抗体to PHD2 / prolyl hydroxylase - C-terminal -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Frmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PHD2/ prolyl hydroxylase (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: Q9GZT9 -
阳性对照
- IHC-Fr: Adult mouse cerebellum tissue. WB: HeLa, untreated and treated with cobalt (II) chloride, whole cell extracts. ICC/IF: DIV9 rat E18 primary cortical neuron, U-87 MG cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226890于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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IHC-Fr |
1/100 - 1/1000.
|
说明 |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
IHC-Fr
1/100 - 1/1000. |
靶标
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功能
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. -
组织特异性
According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. -
疾病相关
Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels. -
序列相似性
Contains 1 Fe2OG dioxygenase domain.
Contains 1 MYND-type zinc finger. - Information by UniProt
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数据库链接
- Entrez Gene: 54583 Human
- Entrez Gene: 112405 Mouse
- Entrez Gene: 308913 Rat
- Omim: 606425 Human
- SwissProt: Q9GZT9 Human
- SwissProt: Q91YE3 Mouse
- SwissProt: P59722 Rat
- Unigene: 444450 Human
see all -
别名
- C1ORF12 antibody
- Chromosome 1 Open Reading Frame 12 antibody
- DKFZp761F179 antibody
see all
图片
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U-87 MG cells stained for PHD2 (green) using ab226890 at 1/500 dilution in ICC/IF.
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IV9 rat E18 primary cortical neuron cells stained for PHD2 (green) using ab226890 at 1/500 dilution in ICC/IF.
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Frozen-sectioned adult mouse cerebellum tissue stained for PHD2 / prolyl hydroxylase using ab226890 at 1/250 dilution in immunohistochemical analysis.
Red: NF-H, stained by NF-H antibody diluted at 1/500.
Blue: DAPI. -
All lanes : Anti-PHD2 / prolyl hydroxylase antibody - C-terminal (ab226890) at 1/2000 dilution
Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 200 µM cobalt (II) chloride for 24 hours whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDa10% SDS-PAGE gel.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226890 尚未被引用在任何文献中。