Anti-PHD2 / prolyl hydroxylase抗体[366G/76/3] (ab103432)
Key features and details
- Mouse monoclonal [366G/76/3] to PHD2 / prolyl hydroxylase
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-PHD2 / prolyl hydroxylase抗体[366G/76/3]
参阅全部 PHD2 / prolyl hydroxylase 一抗 -
描述
小鼠单克隆抗体[366G/76/3] to PHD2 / prolyl hydroxylase -
宿主
Mouse -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- MCF7 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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纯度
Protein A/G purified -
克隆
单克隆 -
克隆编号
366G/76/3 -
骨髓瘤
NS1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab103432于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use 0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. -
组织特异性
According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle. -
疾病相关
Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels. -
序列相似性
Contains 1 Fe2OG dioxygenase domain.
Contains 1 MYND-type zinc finger. - Information by UniProt
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数据库链接
- Entrez Gene: 54583 Human
- Omim: 606425 Human
- SwissProt: Q9GZT9 Human
- Unigene: 444450 Human
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别名
- C1ORF12 antibody
- Chromosome 1 Open Reading Frame 12 antibody
- DKFZp761F179 antibody
see all
图片
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Overlay histogram showing SHSY-5Y cells stained with ab103432 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103432, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab103432 被引用在 4 文献中.
- Hu L et al. TBK1 Is a Synthetic Lethal Target in Cancer with VHL Loss. Cancer Discov 10:460-475 (2020). PubMed: 31810986
- Soilleux EJ et al. Use of novel monoclonal antibodies to determine the expression and distribution of the hypoxia regulatory factors PHD-1, PHD-2, PHD-3 and FIH in normal and neoplastic human tissues. Histopathology 47:602-10 (2005). PubMed: 16324198
- Appelhoff RJ et al. Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the regulation of hypoxia-inducible factor. J Biol Chem 279:38458-65 (2004). PubMed: 15247232
- Stolze IP et al. Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible factor (FIH) in regulating hypoxia-inducible factor (HIF) transcriptional target genes [corrected]. J Biol Chem 279:42719-25 (2004). PubMed: 15302861