Anti-PHD1/prolyl hydroxylase抗体[PHD112/G7] (ab82884)

概述

  • 产品名称Anti-PHD1/prolyl hydroxylase抗体[PHD112/G7]
    参阅全部 PHD1/prolyl hydroxylase 一抗
  • 描述
    小鼠单克隆抗体[PHD112/G7] to PHD1/prolyl hydroxylase
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human protein

  • 阳性对照
    • Human testis tissue

性能

应用

Our Abpromise guarantee covers the use of ab82884 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 47 kDa.
IHC-P Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
  • 组织特异性Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
  • 序列相似性Contains 1 Fe2OG dioxygenase domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp434E026 antibody
    • EGL nine (C.elegans) homolog 2 antibody
    • Egl nine homolog 2 (C. elegans) antibody
    • Egl nine homolog 2 antibody
    • EGLN 2 antibody
    • EGLN2 antibody
    • EGLN2_HUMAN antibody
    • EIT 6 antibody
    • EIT6 antibody
    • Estrogen-induced tag 6 antibody
    • HIF P4H 1 antibody
    • HIF PH1 antibody
    • HIF prolyl hydroxylase 1 antibody
    • HIF-PH1 antibody
    • HIF-prolyl hydroxylase 1 antibody
    • HIFPH 1 antibody
    • HIFPH1 antibody
    • HPH 3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • HPH3 antibody
    • Hypoxia inducible factor prolyl hydroxylase 1 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
    • P4H1 antibody
    • PHD 1 antibody
    • PhD1 antibody
    • prolyl hydroxylase domain containing protein 1 antibody
    • Prolyl hydroxylase domain-containing protein 1 antibody
    see all

Anti-PHD1/prolyl hydroxylase antibody [PHD112/G7] 图像

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded non-small cell type lung tumors with high (top) and low intensities (bottom) for tumor cell expression of PHD1/prolyl hydroxylase.

    PHD1/prolyl hydroxylase was stained using ab82884 at 1/50 dilution, and detected using DAB. Slides were counterstained with hematoxylin to visualize the nuclei.

Anti-PHD1/prolyl hydroxylase antibody [PHD112/G7] (ab82884)参考文献

This product has been referenced in:
  • Andersen S  et al. Overexpression of the HIF Hydroxylases PHD1, PHD2, PHD3 and FIH Are Individually and Collectively Unfavorable Prognosticators for NSCLC Survival. PLoS One 6:e23847 (2011). IHC-P ; Human . Read more (PubMed: 21887331) »

See 1 Publication for this product

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Thank you for your enquiry regarding ab82884.

This particular antibody has not been tested for IHC-P in the lab as standard procedure, however based on this specific publication it is suitable for this application. I would advise you to take a...

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