RabMAb

Anti-PHD1/prolyl hydroxylase抗体[EPR2746] (ab113077)

概述

  • 产品名称Anti-PHD1/prolyl hydroxylase抗体[EPR2746]
    参阅全部 PHD1/prolyl hydroxylase 一抗
  • 描述
    兔单克隆抗体[EPR2746] to PHD1/prolyl hydroxylase
  • 经测试应用适用于: WB, IHC-P, ICC, IPmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human PHD1/prolyl hydroxylase

  • 阳性对照
    • WB: A549, HeLa and MCF-7 cell lysates IHC-P: Human breast carcinoma tissue and Human lung carcinoma tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab113077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min
ICC 1/50 - 1/100.
IP 1/10.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
    • 组织特异性Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
    • 序列相似性Contains 1 Fe2OG dioxygenase domain.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp434E026 antibody
      • EGL nine (C.elegans) homolog 2 antibody
      • Egl nine homolog 2 (C. elegans) antibody
      • Egl nine homolog 2 antibody
      • EGLN 2 antibody
      • EGLN2 antibody
      • EGLN2_HUMAN antibody
      • EIT 6 antibody
      • EIT6 antibody
      • Estrogen-induced tag 6 antibody
      • HIF P4H 1 antibody
      • HIF PH1 antibody
      • HIF prolyl hydroxylase 1 antibody
      • HIF-PH1 antibody
      • HIF-prolyl hydroxylase 1 antibody
      • HIFPH 1 antibody
      • HIFPH1 antibody
      • HPH 3 antibody
      • HPH-1 antibody
      • HPH-3 antibody
      • HPH3 antibody
      • Hypoxia inducible factor prolyl hydroxylase 1 antibody
      • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
      • P4H1 antibody
      • PHD 1 antibody
      • PhD1 antibody
      • prolyl hydroxylase domain containing protein 1 antibody
      • Prolyl hydroxylase domain-containing protein 1 antibody
      see all

    Anti-PHD1/prolyl hydroxylase antibody [EPR2746] 图像

    • All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : MCF-7 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 44 kDa
    • ab113077, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
    • ab113077, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.

    Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077)参考文献

    ab113077 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MKN7, MKN45, SNU-1 cells)
    Gel Running Conditions Reduced Denaturing
    Loading amount 20 µg
    Specification MKN7, MKN45, SNU-1 cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Oct 20 2016

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (10%)
    Sample Human Cell lysate - whole cell (osteosarcoma cells)
    Specification osteosarcoma cells
    Treatment control and siRNA for PHD1
    Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    提交于 Feb 13 2015

    Thank you for contacting us. I would like to reassure you the quality of these antibodies may not have been affected by remaining at 4C during a week.

    We recently undertook an in-depth study examining storage conditions and antibody performa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"