RabMAb

Anti-PHD1/prolyl hydroxylase抗体[EPR2745] (ab108980)

概述

性能

应用

Our Abpromise guarantee covers the use of ab108980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
    • 组织特异性Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
    • 序列相似性Contains 1 Fe2OG dioxygenase domain.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp434E026 antibody
      • EGL nine (C.elegans) homolog 2 antibody
      • Egl nine homolog 2 (C. elegans) antibody
      • Egl nine homolog 2 antibody
      • EGLN 2 antibody
      • EGLN2 antibody
      • EGLN2_HUMAN antibody
      • EIT 6 antibody
      • EIT6 antibody
      • Estrogen-induced tag 6 antibody
      • HIF P4H 1 antibody
      • HIF PH1 antibody
      • HIF prolyl hydroxylase 1 antibody
      • HIF-PH1 antibody
      • HIF-prolyl hydroxylase 1 antibody
      • HIFPH 1 antibody
      • HIFPH1 antibody
      • HPH 3 antibody
      • HPH-1 antibody
      • HPH-3 antibody
      • HPH3 antibody
      • Hypoxia inducible factor prolyl hydroxylase 1 antibody
      • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
      • P4H1 antibody
      • PHD 1 antibody
      • PhD1 antibody
      • prolyl hydroxylase domain containing protein 1 antibody
      • Prolyl hydroxylase domain-containing protein 1 antibody
      see all

    Anti-PHD1/prolyl hydroxylase antibody [EPR2745] 图像

    • All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : MCF-7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 44 kDa
    • Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using ab108980
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108980
    • Immunofluorescent staining of HeLa cells using ab108980
    • Overlay histogram showing HeLa cells stained with ab108980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108980, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980)参考文献

    This product has been referenced in:
    • Lalevée S  et al. miR455 is linked to hypoxia signaling and is deregulated in preeclampsia. Cell Death Dis 5:e1408 (2014). Read more (PubMed: 25188518) »
    • Van Welden S  et al. Differential expression of prolyl hydroxylase 1 in patients with ulcerative colitis versus patients with Crohn's disease/infectious colitis and healthy controls. J Inflamm (Lond) 10:36 (2013). WB, IHC-P ; Human . Read more (PubMed: 24257430) »

    See all 2 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (osteosarcoma cells)
    Specification osteosarcoma cells
    Treatment control and siRNA for PHD1 and GFP-PHD1 overexpression
    Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    提交于 Aug 14 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"