Anti-PHD finger protein 6抗体(ab99398)

概述

  • 产品名称
    Anti-PHD finger protein 6抗体
    参阅全部 PHD finger protein 6 一抗
  • 描述
    兔多克隆抗体to PHD finger protein 6
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 1-50 of Human PHD finger protein 6 (NP_115834.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab99398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May play a role in transcriptional regulation.
  • 组织特异性
    Ubiquitously expressed.
  • 疾病相关
    Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
  • 序列相似性
    Contains 2 PHD-type zinc fingers.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AC004383.6 antibody
    • BFLS antibody
    • BORJ antibody
    • CENP 31 antibody
    • Centromere protein 31 antibody
    • MGC14797 antibody
    • OTTHUMP00000024063 antibody
    • PHD finger protein 6 antibody
    • PHD like zinc finger protein antibody
    • PHD-like zinc finger protein antibody
    • Phf6 antibody
    • PHF6_HUMAN antibody
    see all

Anti-PHD finger protein 6 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PHD finger protein 6 with ab99398 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PHD finger protein 6 antibody (ab99398) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 10 seconds
  • Detection of PHD finger protein 6 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99398 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with exposure time of 3 seconds.

Anti-PHD finger protein 6 antibody (ab99398)参考文献

ab99398 has not yet been referenced specifically in any publications.

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