Anti-PHD finger protein 6抗体(ab99395)


  • 产品名称Anti-PHD finger protein 6抗体
    参阅全部 PHD finger protein 6 一抗
  • 描述
    兔多克隆抗体to PHD finger protein 6
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 315-365 of Human PHD finger protein 6 (NP_115834.1).

  • 阳性对照
    • HeLa and 293T whole cell lysates.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab99395 was affinity purified using an epitope specific to PHD finger protein 6 immobilized on solid support.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域



Our Abpromise guarantee covers the use of ab99395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • 功能May play a role in transcriptional regulation.
  • 组织特异性Ubiquitously expressed.
  • 疾病相关Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
  • 序列相似性Contains 2 PHD-type zinc fingers.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AC004383.6 antibody
    • BFLS antibody
    • BORJ antibody
    • CENP 31 antibody
    • Centromere protein 31 antibody
    • MGC14797 antibody
    • OTTHUMP00000024063 antibody
    • PHD finger protein 6 antibody
    • PHD like zinc finger protein antibody
    • PHD-like zinc finger protein antibody
    • Phf6 antibody
    • PHF6_HUMAN antibody
    see all

Anti-PHD finger protein 6 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling PHD finger protein 6 with ab99395 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PHD finger protein 6 antibody (ab99395) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    developed using the ECL technique

    Predicted band size : 41 kDa

    Exposure time : 3 seconds
  • Detection of PHD finger protein 6 by Western Blot of Immunprecipitate.
    ab99395 at 1µg/ml staining PHD finger protein 6 in HeLa whole cell lysate immunoprecipitated using ab99395 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-PHD finger protein 6 antibody (ab99395)参考文献

ab99395 has not yet been referenced specifically in any publications.

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