概述

  • 产品名称Anti-PHAP1抗体
    参阅全部 PHAP1 一抗
  • 描述
    兔多克隆抗体to PHAP1
  • 特异性This antibody does not cross react with PHAP12a or PHAPIII isoforms.
  • 经测试应用适用于: WB, ICC/IF, Blocking, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human).

  • 阳性对照
    • Raji whole cell lysate.
  • 常规说明Stable for one year.


    Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and –7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-mediated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-mediated cell transformation.

性能

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应用

Our Abpromise guarantee covers the use of ab5991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 32 kDa.Can be blocked with PHAP1 peptide (ab6240).
ICC/IF Use a concentration of 20 µg/ml.
Blocking Use at an assay dependent concentration. PubMed: 16489023
IHC-P Use a concentration of 2 µg/ml.

靶标

  • 功能Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
  • 组织特异性Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
  • 序列相似性Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • 翻译后修饰Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • 细胞定位Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein pp32 antibody
    • AN32A_HUMAN antibody
    • ANP32A antibody
    • C15orf1 antibody
    • Cerebellar leucine rich acidic nuclear protein antibody
    • Hepatopoietin Cn antibody
    • HPPCn antibody
    • I1PP2A antibody
    • Inhibitor 1 of protein phosphatase 2A antibody
    • inhibitor-1 of protein phosphatase-2A antibody
    • Lanp antibody
    • Leucine rich acidic nuclear protein antibody
    • Leucine-rich acidic nuclear protein antibody
    • MAPM antibody
    • Mapmodulin antibody
    • MGC119787 antibody
    • MGC150373 antibody
    • PHAPI antibody
    • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
    • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
    • PP32 antibody
    • Putative HLA DR associated protein I antibody
    • Putative HLA-DR-associated protein I antibody
    • Putative human HLA class II associated protein I antibody
    • Putative human HLA class II-associated protein antibody
    see all

Anti-PHAP1 antibody 图像

  • Western blot analysis of PHAP I expression in human Raji cell (A), mouse (B) and rat (C) testis tissue llysates with anti-PHAP I at 1 µg/ml
  • ab5991 at 2µg/ml staining PHAP1 in mouse small intestine tissue by IHC

  • Immunofluorescence of PHAP I in Mouse Small Intestine cells using ab5991 at 20 ug/ml.

Anti-PHAP1 antibody (ab5991)参考文献

This product has been referenced in:
  • Schafer ZT  et al. Enhanced sensitivity to cytochrome c-induced apoptosis mediated by PHAPI in breast cancer cells. Cancer Res 66:2210-8 (2006). WB, Blocking ; Human . Read more (PubMed: 16489023) »
  • Dirksen EH  et al. Human lymphoblastoid proteome analysis reveals a role for the inhibitor of acetyltransferases complex in DNA double-strand break response. Cancer Res 66:1473-80 (2006). WB ; Human . Read more (PubMed: 16452203) »

See all 2 Publications for this product

Product Wall

The synthetic peptide corresponds to amino acids at carboxy terminus of human PHAP I. Unfortunately, the sequence is not available.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"