概述

  • 产品名称Anti-PHAP1抗体
    参阅全部 PHAP1 一抗
  • 描述
    兔多克隆抗体to PHAP1
  • 经测试应用适用于: WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Chicken, Cow, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: MEMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI, corresponding to N terminal amino acids 1-50 of Human PHAP1

  • 阳性对照
    • Jurkat cell lysate, Human heart.

性能

应用

Our Abpromise guarantee covers the use of ab51013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/312500.

Titre using peptide based assay 1/312500.

靶标

  • 功能Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
  • 组织特异性Expressed in all tissues tested. Highly expressed in kidney and skeletal muscle, moderate levels of expression in brain, placenta and pancreas, and weakly expressed in lung. Found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala.
  • 序列相似性Belongs to the ANP32 family.
    Contains 4 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
  • 翻译后修饰Phosphorylated on serine residues.
    The N-terminus is blocked.
    Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group.
  • 细胞定位Nucleus. Cytoplasm. Endoplasmic reticulum. Translocates to the cytoplasm during the process of neuritogenesis (By similarity). Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • acidic (leucine-rich) nuclear phosphoprotein 32 family, member A antibody
    • Acidic leucine-rich nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein 32 family member A antibody
    • Acidic nuclear phosphoprotein pp32 antibody
    • AN32A_HUMAN antibody
    • ANP32A antibody
    • C15orf1 antibody
    • Cerebellar leucine rich acidic nuclear protein antibody
    • Hepatopoietin Cn antibody
    • HPPCn antibody
    • I1PP2A antibody
    • Inhibitor 1 of protein phosphatase 2A antibody
    • inhibitor-1 of protein phosphatase-2A antibody
    • Lanp antibody
    • Leucine rich acidic nuclear protein antibody
    • Leucine-rich acidic nuclear protein antibody
    • MAPM antibody
    • Mapmodulin antibody
    • MGC119787 antibody
    • MGC150373 antibody
    • PHAPI antibody
    • Potent heat stable protein phosphatase 2A inhibitor I1PP2A antibody
    • Potent heat-stable protein phosphatase 2A inhibitor I1PP2A antibody
    • PP32 antibody
    • Putative HLA DR associated protein I antibody
    • Putative HLA-DR-associated protein I antibody
    • Putative human HLA class II associated protein I antibody
    • Putative human HLA class II-associated protein antibody
    see all

Anti-PHAP1 antibody 图像

  • Lane 1 : Marker
    Lane 2 : Anti-PHAP1 antibody (ab51013) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg/ml with skim milk/ PBS at 5 %

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • ab51013 at 4.0 µg/ml staining myocardial cells from human heart; paraffin embedded; magnification 400x.

Anti-PHAP1 antibody (ab51013)参考文献

ab51013 has not yet been referenced specifically in any publications.

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