参阅全部 PHACTR3 一抗
WB, ELISAmore details
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
Synthetic peptide corresponding to a region within C terminal amino acids 325-374 (QQIEMKLSKRLSQRPAVEELERRNILKQRNDQTEQEERREIKQRLTRKL N) of Human PHACTR3 (NP_ 899069).
- 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- 存储溶液Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- 纯度Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/12500.
- 组织特异性Abundantly expressed in brain. Also found in several tumors such as lung carcinomas, nervous tumors and HL-60 leukemia cells. Isoform 3 is the major form in U937, GOTO and HL-60 leukemia cells.
- 序列相似性Belongs to the phosphatase and actin regulator family.
Contains 4 RPEL repeats.
- 细胞定位Nucleus matrix. Localized to the nuclear matrix-intermediate filament scaffold. Isoform 2 is also found in some cytoplasmic extensions.
Information by UniProt
- C20orf101 antibody
- H17739 antibody
- MGC117178 antibody
Anti-PHACTR3 antibody 图像
Western blot - PHACTR3 antibody (ab81340)
Anti-PHACTR3 antibody (ab81340) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 62 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?)
Additional bands at : 38 kDa (possible isoform),39 kDa (possible isoform).
Anti-PHACTR3 antibody (ab81340)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"