Anti-PGP9.5抗体- Neuronal Marker (ab10410)


  • 产品名称Anti-PGP9.5抗体- Neuronal Marker
    参阅全部 PGP9.5 一抗
  • 描述
    豚鼠多克隆抗体to PGP9.5 - Neuronal Marker
  • 经测试应用适用于: IHC-Fr, IHC-P, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Macaque Monkey
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 175-191 of soluble cytoplasmic protein PGP9.5, human.

  • 阳性对照
    • IF: DU145 cell line



Our Abpromise guarantee covers the use of ab10410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/50 - 1/500.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr 1/50 - 1/500.
ICC/IF 1/200.


  • 功能Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • 组织特异性Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • 疾病相关Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • 序列相似性Belongs to the peptidase C12 family.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody - Neuronal Marker 图像

  • ab10410, at a dilution of 1/500, staining PGP9.5 in rat submucous plexus by Immunohistochemistry (PFA perfusion fixed frozen sections).
  • ICC/IF image of ab10410 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10410, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96959, DyLight® 488 goat anti-guinea pig IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PGP9.5 antibody - Neuronal Marker (ab10410)参考文献

This product has been referenced in:
  • Buckinx R  et al. Transient receptor potential vanilloid type 1 channel (TRPV1) immunolocalization in the murine enteric nervous system is affected by the targeted C-terminal epitope of the applied antibody. J Histochem Cytochem 61:421-32 (2013). Read more (PubMed: 23482327) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Sample Rat Tissue sections (Dorsal root ganglion)
Specification Dorsal root ganglion
Permeabilization Yes - 0,1% Tween -20
Fixative Paraformaldehyde

Yulia Sidorova

Verified customer

提交于 Feb 03 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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As I mentioned in our phone call, your free of charge replacement for ab10410 should have been delivered today, 31st Oct 2011. If there is anything else I can help you with, please let me know.