- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-PGP9.5抗体[EPR4117]
参阅全部 PGP9.5 一抗 -
描述兔单克隆抗体[EPR4117] to PGP9.5
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宿主Rabbit
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经测试应用适用于: WB, IP, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide within Human PGP9.5 aa 50-150. The exact sequence is proprietary.
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阳性对照
- Fetal brain, Y79, U-87 MG, HAP1 and SH SY5Y lysates; Human brain tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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解离常数（KD）KD = 7.20 x 10 -10 M Learn more about KD
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR4117
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab109261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 24 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/100. Perform antigen retrieval before commencing with IHC staining protocol |
靶标
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功能Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
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组织特异性Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
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疾病相关Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
序列相似性Belongs to the peptidase C12 family.
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翻译后修饰O-glycosylated.
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细胞定位Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
- Information by UniProt
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数据库链接
- Entrez Gene: 7345 Human
- Entrez Gene: 22223 Mouse
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: P09936 Human
- SwissProt: Q9R0P9 Mouse
- SwissProt: Q00981 Rat
- Unigene: 518731 Human
see all -
别名
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
图片
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![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](http://www.abcam.cn/ps/products/109/ab109261/Images/ab109261-279567-ab109261KOWB.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: UCHL1 (KO) knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab109261 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
Ab109261 was shown to specifically react with UCHL1 (KO) in wild-type cells as signal was lost in UCHL1 (KO) knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. Ab109261 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)](http://www.abcam.cn/ps/products/109/ab109261/Images/PGP95-Primary-antibodies-ab109261-2.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PGP9.5 antibody [EPR4117] (ab109261)ab109261 at 1/50 dilution staining PGP9.5 in Human brain by Immunohistochemistry, Paraffin-embedded tissue. -
![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](http://www.abcam.cn/ps/products/109/ab109261/Images/PGP95-Primary-antibodies-ab109261-1.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Y79 lysate
Lane 3 : U-87 MG lysate
Lane 4 : SH SY5Y lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa -
![Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)](http://www.abcam.cn/ps/products/109/ab109261/Images/PGP95-Primary-antibodies-ab109261-12.jpg)
Western blot - Anti-PGP9.5 antibody [EPR4117] (ab109261)Image courtesy of Dr Simone Diestel by Abreview.All lanes : Anti-PGP9.5 antibody [EPR4117] (ab109261) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from adult mouse brain homogenate (CD-1 strain)
Lane 2 : Whole tissue lysate prepared from non transfected control cells (low endogenous expression of UCH-L1)
Lane 3 : Whole tissue lysate prepared from cells transfected with UCH-L1-Flag cDNA (positive control)
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabiit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 2 minutes
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![Other - Anti-PGP9.5 antibody [EPR4117] (ab109261)](http://www.abcam.cn/ps/products/109/ab109261/Images/PGP95-Primary-antibodies-ab109261-14.jpg)
Other - Anti-PGP9.5 antibody [EPR4117] (ab109261)
实验方案
文献
This product has been referenced in:
- Purmessur D et al. Notochordal cell-derived therapeutic strategies for discogenic back pain. Global Spine J 3:201-18 (2013). IHC . Read more (PubMed: 24436871) »
客户评价及客户问答
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