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Our Abpromise guarantee covers the use of ab10404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 26.8 kDa.Can be blocked with Human PGP9.5 peptide (ab38203).|
|IHC-P||1/100 - 1/300.|
|IHC (PFA fixed)||1/200. PubMed: 17508253|
|ICC||Use at an assay dependent concentration.|
ab10404 at a dilution of 1/1000, detecting PGP9.5 in cortical neurons (Alexa 488 secondary at 1/2000) on rat brain tissue sections prepared for free floating IHC (4% PFA fixed, 30
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
ab10404 staining PGP9.5 in Mouse Endometriotic tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in 3% BSA) for 20 hours at 4°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.