概述

  • 产品名称Anti-PGLS抗体
    参阅全部 PGLS 一抗
  • 描述
    兔多克隆抗体to PGLS
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 208-257 (AAVLKRILED QEENPLPAAL VQPHTGKLCW FLDEAAARLL TVPFEKHSTL ) of human PGLS (NP_036220).

  • 阳性对照
    • 721_B cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-PGLS antibody 图像

  • Anti-PGLS antibody (ab83867) at 1 µg/ml (5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab83867 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83867, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab83867 at 5 µg/ml staining PGLS in Human tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections.
  • ab83867 at 5 µg/ml staining PGLS in Human colon tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PGLS antibody (ab83867)参考文献

ab83867 has not yet been referenced specifically in any publications.

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