Anti-PGLS抗体(ab229980)
Key features and details
- Rabbit polyclonal to PGLS
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PGLS抗体
参阅全部 PGLS 一抗 -
描述
兔多克隆抗体to PGLS -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Cow -
免疫原
Recombinant full length protein corresponding to Human PGLS aa 1 to the C-terminus.
Database link: O95336 -
阳性对照
- WB: Mouse liver and brain lysates; U-251 MG, THP-1, HepG2 and HeLa whole cell lysates. IHC-P: Human thyroid and tonsil tissues. IP: U-251 MG whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab229980于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/20 - 1/200.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
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IP |
1/200 - 1/2000.
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说明 |
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IHC-P
1/20 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 28 kDa. |
IP
1/200 - 1/2000. |
靶标
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相关性
PGLS belongs to the glucosamine/galactosamine-6-phosphate isomerase family, 6-phosphogluconolactonase subfamily. It is implicated in the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 616120 Cow
- Entrez Gene: 25796 Human
- Entrez Gene: 66171 Mouse
- Omim: 604951 Human
- SwissProt: Q2TBQ8 Cow
- SwissProt: O95336 Human
- SwissProt: Q9CQ60 Mouse
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别名
- 6 phosphogluconolactonase antibody
- 6PGL antibody
图片
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All lanes : Anti-PGLS antibody (ab229980) at 1/1000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
Lane 3 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28,52 kDa why is the actual band size different from the predicted? -
Paraffin-embedded human thyroid tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human tonsil tissue stained for PGLS using ab229980 at 1/100 dilution in immunohistochemical analysis.
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PGLS was immunoprecipitated from 500 µg U-251 MG (human brain glioma cell line) whole cell lysate with 4 µg ab229980.
Lane 1: Rabbit monoclonal IgG (1 µg) IP in U-251 MG whole cell lysate.
Lane 2: ab229980 IP in U-251 MG whole cell lysate.
Lane 3: U-251 MG whole cell lysate 20 µg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab229980 尚未被引用在任何文献中。