Anti-PGK1抗体[14] (ab67335)


  • 产品名称Anti-PGK1抗体[14]
    参阅全部 PGK1 一抗
  • 描述
    小鼠单克隆抗体[14] to PGK1
  • 经测试应用适用于: IHC-P, Flow Cyt, WB, ELISAmore details
  • 种属反应性
    与反应: Human, Recombinant Fragment
  • 免疫原

    Recombinant full length protein (Human) expressed in E. coli.



Our Abpromise guarantee covers the use of ab67335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA Use at an assay dependent concentration.


  • 功能In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • 疾病相关Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • 序列相似性Belongs to the phosphoglycerate kinase family.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell migration-inducing gene 10 protein antibody
    • Epididymis secretory sperm binding protein Li 68p antibody
    • HEL S 68p antibody
    • MGC117307 antibody
    • MGC8947 antibody
    • MIG10 antibody
    • pgk1 antibody
    • PGK1_HUMAN antibody
    • PGKA antibody
    • Phosphoglycerate kinase 1 antibody
    • Primer recognition protein 2 antibody
    • PRP 2 antibody
    see all

Anti-PGK1 antibody [14] 图像

  • Anti-PGK1 antibody [14] (ab67335) at 1/1000 dilution + Human recombinant PGK1 protein

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Overlay histogram showing HeLa cells stained with ab67335 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab67335, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Hela cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PGK1 antibody [14] (ab67335)参考文献

ab67335 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Formaldehyde
Permeabilization Yes - BD Perm/Wash Buffer
Sample Human Cell (PBMC)
Specification PBMC
Gating Strategy FSC-A/SSC-A
Preparation Cell harvesting/tissue preparation method: Ficoll isolation of PBMC from whole blood, spin down, wash in FACS buffer 1x, fix, wash 2x, and stain with primary antibody overnight.
Sample buffer: Buffer composition at analysis was 0.1% sodium azide with FBS in phosphate buffered solution.

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提交于 Jul 04 2014