Anti-PGC1 alpha + beta抗体(ab72230)

概述

  • 产品名称Anti-PGC1 alpha + beta抗体
    参阅全部 PGC1 alpha + beta 一抗
  • 描述
    兔多克隆抗体to PGC1 alpha + beta
  • 特异性The immunogen design for ab72230 is shown to cross-react with both PGC1 alpha (ID: 124782 and Swissprot Q9UBK2) and PGC1 beta (ID: 240776 and SwissProt Q86YN6).
  • 经测试应用适用于: ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Cow, Dog, Pig, Rhesus monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human PGC1 alpha.

    (Peptide available as ab73600.)

  • 阳性对照
    • This antibody gave a positive signal in the following Tissue Lysates: Human Skeletal Muscle Mouse Testis Mouse Colon Mouse Skeletal Muscle Rat Ovary Rat Thymus
  • 常规说明

    PGC1 alpha has a predicted MW of 92 kDa and PGC1 beta has a predicted MW of 113 kDa.

     

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

     

应用

Our Abpromise guarantee covers the use of ab72230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).
ICC/IF 1/100.

靶标

  • 相关性PGC1 alpha - The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene). PGC1 beta - Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure.
  • 细胞定位Nucleus
  • 数据库链接
  • 别名
    • LEM6 antibody
    • Ligand effect modulator 6 antibody
    • PERC antibody
    • Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha antibody
    • Peroxisome proliferative activated receptor, gamma, coactivator 1 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha antibody
    • peroxisome proliferator-activated receptor gamma coactivator 1 beta antibody
    • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha antibody
    • Peroxisome proliferator-activated receptor gamma coactivator 1-beta antibody
    • peroxisome proliferator-activated receptor gamma, coactivator 1 beta antibody
    • PGC 1 (alpha) antibody
    • PGC 1 alpha antibody
    • PGC 1v antibody
    • PGC-1(beta) antibody
    • PGC-1-alpha antibody
    • PGC-1-beta antibody
    • PGC-1-related estrogen receptor alpha coactivator antibody
    • PGC1 antibody
    • PGC1(alpha) antibody
    • PGC1A antibody
    • PGC1v antibody
    • PPAR gamma coactivator 1 alpha antibody
    • PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 antibody
    • PPAR gamma coactivator 1 antibody
    • PPAR gamma coactivator variant form antibody
    • PPAR gamma coactivator-1beta antibody
    • PPAR-gamma coactivator 1-alpha antibody
    • PPAR-gamma coactivator 1-beta antibody
    • PPARGC 1 alpha antibody
    • PPARGC-1-alpha antibody
    • PPARGC-1-beta antibody
    • PPARGC1 antibody
    • PPARGC1A antibody
    • Ppargc1b antibody
    • PRGC1_HUMAN antibody
    • PRGC2_HUMAN antibody
    see all

Anti-PGC1 alpha + beta antibody 图像

  • Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling PGC1 alpha+beta with ab72230 at a 1/100 dilution showing nuclear staining. Permeabilisation using 0.1% tritonX-100.

    Secondary ab: Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/1000 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab72230 at 1/100 dilution, Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. -ve control 2 is ab7291 at 1/1000 dilution, Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.

  • All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 3 : Ovary (Rat) Tissue Lysate
    Lane 4 : Colon (Mouse) Tissue Lysate
    Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
    Lane 6 : Thymus (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 91 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,45 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
    We have occasionally observed bands in the region of 90 kDa in batches of this antibody, which corresponds to the molecular weight based on primary sequence.
  • All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Colon (Mouse) Tissue Lysate
    Lane 3 : Thymus (Rat) Tissue Lysate
    Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
    Lane 5 : Colon (Mouse) Tissue Lysate with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
    Lane 6 : Thymus (Rat) Tissue Lysate with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
    Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330) with PGC1 beta peptide at 1 µg/ml
    Lane 8 : Colon (Mouse) Tissue Lysate with PGC1 beta peptide at 1 µg/ml
    Lane 9 : Thymus (Rat) Tissue Lysate with PGC1 beta peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 91 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,73 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    Western Blot showing equal blocking with the PGC1 alpha and PGC1 beta peptides.
  • ab72230 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.

    Ab72230 gave a positive result in ELISA against PGC1 alpha peptide and PGC1 beta peptide. This indicates that it recognises both forms.

    Orange line: ELISA against immunising peptide ab73600
    Yellow line: ELISA against ab88232

     

Anti-PGC1 alpha + beta antibody (ab72230)参考文献

This product has been referenced in:
  • Jiao J  et al. Chronic leucine supplementation improves lipid metabolism in C57BL/6J mice fed with a high-fat/cholesterol diet. Food Nutr Res 60:31304 (2016). WB ; Mouse . Read more (PubMed: 27616737) »
  • Al-Rewashdy H  et al. Utrophin A is essential in mediating the functional adaptations of mdx mouse muscle following chronic AMPK activation. Hum Mol Genet 24:1243-55 (2015). Read more (PubMed: 25324540) »

See all 8 Publications for this product

Product Wall

Thank you for your enquiry.

According to the Uniprot protein database page for this protein, the PGC1 beta has several isoforms at different molecular weights, including several at around 110 kDa. So I can suggest the band at 110 kDa is the ...

Read More

Thanks for your enquiry.

If you could please provide the lot number for this vial of ab54481, I will look into the concentration and follow up with you.

Thanks in advance for the additional information!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Frontal cortex)
Loading amount 20 µg
Specification Frontal cortex
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 13 2012

Thank you for contacting us.

These antibodies can be diluted either in TBST + 3% BSA/Milk.

The membrane can be blocked using either 5% BSA or 5% Milk. Milk is cheaper than BSA so if milk give high background we always recommend our...

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Thank you for contacting us and your interest in our products.


Anti-PGC1 alpha antibody (ab77210) has to our knowledgenot yetbeen tested with rat samples. However, the immunogen used (residues 649-799 of human PGC1 alpha, SwissProt refe...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - nuclear (liver)
Loading amount 50 µg
Specification liver
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 12 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"