概述

  • 产品名称Anti-PGAM1抗体
    参阅全部 PGAM1 一抗
  • 描述
    兔多克隆抗体to PGAM1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 16-209 of Human PGAM1 (AAH00455).

  • 阳性对照
    • 293T, A431, Jurkat and Raji cell lysates

性能

应用

Our Abpromise guarantee covers the use of ab96622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.

靶标

  • 功能Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
  • 组织特异性In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
  • 序列相似性Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BPG dependent PGAM 1 antibody
    • BPG dependent PGAM1 antibody
    • BPG-dependent PGAM 1 antibody
    • OTTHUMP00000059414 antibody
    • PGAM 1 antibody
    • PGAM A antibody
    • PGAM B antibody
    • PGAM-B antibody
    • PGAM1 antibody
    • PGAM1_HUMAN antibody
    • PGAMA antibody
    • PGAMB antibody
    • Phosphoglycerate mutase 1 antibody
    • Phosphoglycerate mutase 1 brain antibody
    • Phosphoglycerate mutase A antibody
    • Phosphoglycerate mutase A nonmuscle form antibody
    • Phosphoglycerate mutase isozyme B antibody
    see all

Anti-PGAM1 antibody 图像

  • All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
  • All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution

    Lane 1 : Recombinant Human PGAM1 protein (ab100885) at 0.1 µg
    Lane 2 : Recombinant Human PGAM1 protein (ab100885) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Anti-PGAM1 antibody (ab96622)参考文献

ab96622 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat Cells)
Loading amount 30 µg
Specification Jurkat Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Aug 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"